| Author | Tang, Thuy-Huong Ta | |
| Author | López-Vélez, Rogelio | |
| Author | Lanza, Marta | |
| Author | Shelley, Anthony John | |
| Author | Rubio, Jose Miguel | |
| Author | Luz, Sérgio Luiz Bessa | |
| Access date | 2011-03-31T18:49:14Z | |
| Available date | 2011-03-31T18:49:14Z | |
| Document date | 2010 | |
| Citation | TANG, Thuy-Huong Ta et al. Nested PCR to detect and distinguish the sympatric filarial species Onchocerca volvulus, Mansonella ozzardi and Mansonella perstans in the Amazon Region. Memória do Instituto Oswaldo Cruz, Rio de Janeiro, v. 105, n. 6, p. 823-828, Sept. 2010. | pt_BR |
| ISSN | 0074-0276 | |
| URI | https://www.arca.fiocruz.br/handle/icict/1811 | |
| Language | eng | pt_BR |
| Rights | open access | pt_BR |
| Title | Nested PCR to detect and distinguish the sympatric filarial species Onchocerca volvulus, Mansonella ozzardi and Mansonella perstans in the Amazon Region | pt_BR |
| Type | Article | pt_BR |
| Place of Defense | Rio de Janeiro | pt_BR |
| Abstract | We present filaria-nested polymerase chain reaction (PCR), which is based on amplification of first internal transcribed spacer rDNA to distinguish three parasitic filarial species (Onchocerca volvulus, Mansonella ozzardi and Mansonella perstans) that can be found in the Amazon Region. Nested PCR-based identifications yielded the same results as those utilizing morphological characters. Nested PCR is highly sensitive and specific and it detects low-level infections in both humans and vectors. No cross-amplifications were observed with various other blood parasites and no false-positive results were obtained with the nested PCR. The method works efficiently with whole-blood, blood-spot and skin biopsy samples. Our method may thus be suitable for assessing the efficacy of filaria control programmes in Amazonia by recording parasite infections in both the human host and the vector. By specifically
differentiating the major sympatric species of filaria, this technique could also enhance epidemiological research in the region. | pt_BR |
| Affilliation | Instituto de Salud Carlos III. Centro Nacional de Microbiología. Madrid, España. | pt_BR |
| Affilliation | Hospital Ramón y Cajal. Unidad de Medicina Tropical y Parasitología Clínica. Servicio de Enfermedades Infecciosas. Madrid, España. | pt_BR |
| Affilliation | Instituto de Salud Carlos III. Centro Nacional de Microbiología. Madrid, España. | pt_BR |
| Affilliation | Natural History Museum. Department of Entomology. London, UK. | pt_BR |
| Affilliation | Instituto de Salud Carlos III. Centro Nacional de Microbiología. Madrid, España. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Manaus, AM, Brasil. | pt_BR |
| Subject | Nested PCR | pt_BR |
| Subject | 18S rDNA | pt_BR |
| Subject | ITS-1 | pt_BR |
| Subject | Onchocerciasis | pt_BR |
| Subject | Mansonelliasis | pt_BR |
| Subject | Brazil | pt_BR |
