Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/1811
Title: Nested PCR to detect and distinguish the sympatric filarial species Onchocerca volvulus, Mansonella ozzardi and Mansonella perstans in the Amazon Region
Authors: Tang, Thuy-Huong Ta
López-Vélez, Rogelio
Lanza, Marta
Shelley, Anthony John
Rubio, Jose Miguel
Luz, Sérgio Luiz Bessa
Affilliation: Instituto de Salud Carlos III. Centro Nacional de Microbiología. Madrid, España.
Hospital Ramón y Cajal. Unidad de Medicina Tropical y Parasitología Clínica. Servicio de Enfermedades Infecciosas. Madrid, España.
Instituto de Salud Carlos III. Centro Nacional de Microbiología. Madrid, España.
Natural History Museum. Department of Entomology. London, UK.
Instituto de Salud Carlos III. Centro Nacional de Microbiología. Madrid, España.
Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Manaus, AM, Brasil.
Abstract: We present filaria-nested polymerase chain reaction (PCR), which is based on amplification of first internal transcribed spacer rDNA to distinguish three parasitic filarial species (Onchocerca volvulus, Mansonella ozzardi and Mansonella perstans) that can be found in the Amazon Region. Nested PCR-based identifications yielded the same results as those utilizing morphological characters. Nested PCR is highly sensitive and specific and it detects low-level infections in both humans and vectors. No cross-amplifications were observed with various other blood parasites and no false-positive results were obtained with the nested PCR. The method works efficiently with whole-blood, blood-spot and skin biopsy samples. Our method may thus be suitable for assessing the efficacy of filaria control programmes in Amazonia by recording parasite infections in both the human host and the vector. By specifically differentiating the major sympatric species of filaria, this technique could also enhance epidemiological research in the region.
Keywords: Nested PCR
18S rDNA
ITS-1
Onchocerciasis
Mansonelliasis
Brazil
Issue Date: 2010
Citation: TANG, Thuy-Huong Ta et al. Nested PCR to detect and distinguish the sympatric filarial species Onchocerca volvulus, Mansonella ozzardi and Mansonella perstans in the Amazon Region. Memória do Instituto Oswaldo Cruz, Rio de Janeiro, v. 105, n. 6, p. 823-828, Sept. 2010.
Place of defense: Rio de Janeiro
ISSN: 0074-0276
Copyright: open access
Appears in Collections:AM - ILMD - Artigos de Periódicos

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