Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/18177
Title: Leishmania infection inhibits macrophage motility by altering F-actin dynamics and the expression of adhesion complex proteins
Authors: Menezes, Juliana Perrone Bezerra de
Koushik, Amrita
Das, Satarupa
Guven, Can
Siegel, Ariel
Silva, Maria Fernanda Laranjeira
Losert, Wolfgang
Andrews, Norma W
Affilliation: University of Maryland. Department of Cell Biology and Molecular Genetics. Maryland, USA / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil
University of Maryland. Department of Cell Biology and Molecular Genetics. Maryland, USA
University of Maryland. Department of Physics. Maryland, USA
University of Maryland. Department of Physics. Maryland, USA
University of Maryland. Department of Cell Biology and Molecular Genetics. Maryland, USA
University of Maryland. Department of Cell Biology and Molecular Genetics. Maryland, USA
University of Maryland. Department of Physics. Maryland, USA
University of Maryland. Department of Cell Biology and Molecular Genetics. Maryland, USA
Abstract: Leishmania is an intracellular protozoan parasite that causes a broad spectrum of clinical manifestations, ranging from self-healing skin lesions to fatal visceralizing disease. As the host cells of choice for all species of Leishmania, macrophages are critical for the establishment of infections. How macrophages contribute to parasite homing to specific tissues and how parasites modulate macrophage function are still poorly understood. In this study, we show that Leishmania amazonensis infection inhibits macrophage roaming motility. The reduction in macrophage speed is not dependent on particle load or on factors released by infected macrophages. L. amazonensis-infected macrophages also show reduced directional migration in response to the chemokine MCP-1. We found that infected macrophages have lower levels of total paxillin, phosphorylated paxillin, and phosphorylated focal adhesion kinase when compared to noninfected macrophages, indicating abnormalities in the formation of signaling adhesion complexes that regulate motility. Analysis of the dynamics of actin polymerization at peripheral sites also revealed a markedly enhanced F-actin turnover frequency in L. amazonensis-infected macrophages. Thus, Leishmania infection inhibits macrophage motility by altering actin dynamics and impairing the expression of proteins that function in plasma membrane-extracellular matrix interactions.
Keywords: Leishmania
Macrophages
Leishmania amazonensis
infection
Proteins
Skin
keywords: Leishmania
Macrófagos
Leishmania amazonensis
Infecção
Proteínas
Pele
Issue Date: 2017
Publisher: Wiley
Citation: MENEZES, J. P. B. et al. Leishmania infection inhibits macrophage motility by altering F-actin dynamics and the expression of adhesion complex proteins. Cellular Microbiology, v. 19, p. e12668, 2017.
DOI: 10.1111/cmi.12668
ISSN: 1462-5814
Copyright: open access
Appears in Collections:IGM - Artigos de Periódicos

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