| Author | Pereira, Paulo Rogério | |
| Author | Oliveira Junior, Manoel Carneiro | |
| Author | Mackenzie, Breanne | |
| Author | Chiovatto, Jaime Eduardo Davino | |
| Author | Matos, Yves | |
| Author | Greiffo, Flavia Regina | |
| Author | Oliveira, Nicole Cristine Rigonato | |
| Author | Brugemman, Thayse Regina | |
| Author | Delle, Humberto | |
| Author | Idzko, Marco | |
| Author | Albertini, Regiane | |
| Author | Oliveira, Ana Paula Ligeiro | |
| Author | Rodrigues, Nilsa Regina Damasceno | |
| Author | Caldini, Elia Garcia | |
| Author | Fernandez, Isis Ensil | |
| Author | Faria Neto, Hugo Caire Castro | |
| Author | Dolhnikoff, Marisa | |
| Author | Eickelberg, Oliver | |
| Author | Vieira, Rodolfo Paula | |
| Access date | 2017-04-04T15:46:13Z | |
| Available date | 2017-04-04T15:46:13Z | |
| Document date | 2016 | |
| Citation | PEREIRA, Paulo Rogerio; et al. Exercise Reduces Lung Fibrosis Involving Serotonin/Akt Signaling. Medicine, Science in Sports & Exercise, v.48, n.7, p.1276-1284, July 2016. | pt_BR |
| ISSN | 0195-9131 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/18261 | |
| Language | eng | pt_BR |
| Publisher | American College of Sports Medicine | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Treinamento aeróbico | pt_BR |
| Subject in Portuguese | Fibrose | pt_BR |
| Subject in Portuguese | Exercício | pt_BR |
| Subject in Portuguese | Inflamação | pt_BR |
| Title | Exercise Reduces Lung Fibrosis Involving Serotonin/Akt Signaling | pt_BR |
| Type | Article | |
| DOI | 10.1249/MSS.0000000000000907 | |
| Abstract | PURPOSE:
Idiopathic pulmonary fibrosis (IPF) is a chronic fibrosing interstitial pneumonia, which involves aberrant serotonin (5-hydroxytryptamine [5-HT]) and Akt signaling. As protective effects of chronic aerobic training (AT) have been demonstrated in the context of lung injury, this study investigated whether AT attenuates bleomycin-induced lung fibrosis partly via a reduction of 5-HT and AKT signaling.
METHODS:
Seventy-two C57BL/6 male mice were distributed in Control (Co), Exercise (Ex), Fibrosis (Fi), and Fibrosis + Exercise (Fi + Ex) groups. Bleomycin (1.5 UI·kg) was administered on day 1 and treadmill AT began on day 15 and continued for 60 min·d, 5 d·wk for 4 wk. We evaluated total and differential cell counts in bronchoalveolar lavage (BAL), interleukin (IL)-1β, IL-6, CXCL1/KC, IL-10, tumor necrosis factor α, and transforming growth factor β levels in BAL, collagen content in lung parenchyma, 5-HT levels in BAL fluid and in serum, the expression of 5-HT2B receptor, and Akt phosphorylation in lung tissue.
RESULTS:
AT reduced bleomycin-increased number of total cells (P < 0.001), neutrophils (P < 0.01), macrophages (P < 0.01), and lymphocytes (P < 0.05) in BAL. It also reduced the levels of IL-1β (P < 0.01), IL-6 (P < 0.05), CXCL1/KC (P < 0.001), tumor necrosis factor α (P < 0.001), and transforming growth factor β (P < 0.001). It increased expression of ant-inflammatory cytokine IL-10 (P < 0.001). It reduced bleomycin-increased 5-HT levels in BAL (P < 0.001) and in serum (P < 0.05). Reductions in collagen fiber deposition (P < 0.01), 5-HT2B receptor expression (P < 0.01), and Akt phosphorylation in lung tissue were observed.
CONCLUSIONS:
AT accelerates the resolution of lung inflammation and fibrosis in a model of bleomycin-induced lung fibrosis partly via attenuation of 5-HT/Akt signaling. | pt_BR |
| Affilliation | Universidade Nove de Julho. Laboratório de Imunologia Pulmonar e do Exercício. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Universidade Nove de Julho. Laboratório de Imunologia Pulmonar e do Exercício. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Universidade Nove de Julho. Laboratório de Imunologia Pulmonar e do Exercício. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Universidade Nove de Julho. Laboratório de Imunologia Pulmonar e do Exercício. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Universidade Nove de Julho. Laboratório de Imunologia Pulmonar e do Exercício. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Universidade Nove de Julho. Laboratório de Imunologia Pulmonar e do Exercício. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Universidade Nove de Julho. Laboratório de Imunologia Pulmonar e do Exercício. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Universidade de São Paulo. Escola de Medicina. Laboratório de Terapêutica Experimental. São Paulo, SP, Bbrasil. | pt_BR |
| Affilliation | Universidade Nove de Julho. Laboratório de Imunologia Pulmonar e do Exercício. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | University Hospital Freiburg. Department of Pneumology. COPD and Asthma Research Group. Freiburg, Germany. | pt_BR |
| Affilliation | Universidade Nove de Julho. Laboratório de Imunologia Pulmonar e do Exercício. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Universidade Nove de Julho. Laboratório de Imunologia Pulmonar e do Exercício. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Universidade de São Paulo. Escola de Medicina. Laboratório de Biologia Celular. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Universidade de São Paulo. Escola de Medicina. Laboratório de Biologia Celular. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | University Hospital of the Ludwig Maximilians University Munich. Comprehensive Pneumology center. Munich, Germany. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Universidade de São Paulo. Escola de Medicina. Departamento de Patologia. Laboratório de Poluição Atmosférica Experimental (LPAE). São Paulo, SP, Brasil. | pt_BR |
| Affilliation | University Hospital of the Ludwig Maximilians University Munich. Comprehensive Pneumology center. Munich, Germany. | pt_BR |
| Affilliation | Universidade Nove de Julho. Laboratório de Imunologia Pulmonar e do Exercício. São Paulo, SP, Brasil. | pt_BR |
| Subject | Aerobic training | pt_BR |
| Subject | Fibrosis | pt_BR |
| Subject | Exercise | pt_BR |
| Subject | Inflammation | pt_BR |
| Subject | 5-HT | pt_BR |
| e-ISSN | 1530-0315 | |
| Embargo date | 2030-01-01 | |
