Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/18701
Title: Genetic polymorphism in Leishmania (Viannia) braziliensis detected in mucosal leishmaniasis of HIV-infected and non-HIV-infected patients
Authors: Oliveira, Fernanda Santos de
Valete-Rosalino, Cláudia Maria
Schubach, Armando de Oliveira
Madeira, Maria de Fátima
Pacheco, Raquel da Silva
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Sistemática Bioquímica. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Vigilância em Leishmanioses. Rio de Janeiro, RJ. Brasil / Universidade Federal do Rio de Janeiro. Faculdade de Medicina. Departamento de Otorrinolaringologia e Oftalmologia. Ri de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Vigilância em Leishmanioses. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Vigilância em Leishmanioses. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Sistemática Bioquímica. Rio de Janeiro, RJ. Brasil.
Abstract: The genetic polymorphism of Leishmania (Viannia) braziliensis detected in cases of mucosal leishmaniasis (ML) from HIV-infected and non HIV-infected patients was evaluated. Nine samples from three HIV-infected patients and five samples from five non HIV-infected patients were analysed by polymerase chain reaction (PCR), low-stringency single-specific primer PCR (LSSP-PCR) and phenetic analysis. The presence of L. (V.) braziliensis DNA was detected in all samples by specific PCR assay. The intraspecific polymorphism of the variable region of L. (V.) braziliensis kDNA minicircles was investigated by LSSP-PCR. Phenetic analysis grouped the genetic profiles into two distinct clusters, which discriminated between samples obtained from HIV-infected and non HIV-infected patients. In two HIV-infected patients, identical genetic profiles were detected in lesions biopsied at different times after the treatment of the initial lesion. Interestingly, genetically divergent profiles were detected in the cutaneous and mucosal lesions of the same HIV-infected patient collected at the same time. This is the first work comparing genetic polymorphism of L. (V.) braziliensis in cases of mucosal leishmaniasis from HIV-infected and non HIV-infected patients.
Keywords: Mucosal leishmaniasis
Leishmania/HIV co-infection
Leishmania (Viannia) braziliensis
PCR
LSSP-PCR
keywords: Leishmaniose mucosa
Leishmania
HIV
Co-ínfecção
Reação em Cadeia da Polimerase
Leishmania braziliensis
Issue Date: 2012
Publisher: Elsevier
Citation: OLIVEIRA, Fernanda Santos de; et al. Genetic polymorphism in Leishmania (Viannia) braziliensis detected in mucosal leishmaniasis of HIV-infected and non-HIV-infected patients. Transactions of the Royal Society of Tropical Medicine and Hygiene, v.106, p.683– 687, 2012.
DOI: 10.1016/j.trstmh.2012.07.007
ISSN: 0035-9203
Copyright: restricted access
Appears in Collections:IOC - Artigos de Periódicos

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