Author | Gomes, Cinthia Bernardes | |
Author | Silva, Franklin Souza | |
Author | Charret, Karen dos Santos | |
Author | Pereira, Bernardo Acácio Santini | |
Author | Finkelstein, Léa Cysne | |
Author | Souza, Raquel Santos de | |
Author | Côrtes, Luzia Monteiro de Castro | |
Author | Pereira, Mirian Claudia Souza | |
Author | Oliveira, Francisco Odêncio Rodrigues de | |
Author | Alves, Carlos Roberto | |
Access date | 2017-05-04T12:40:56Z | |
Available date | 2017-05-04T12:40:56Z | |
Document date | 2017 | |
Citation | GOMES, Cinthia Bernardes; et al. Increasing in cysteine proteinase B expression and enzymatic activity during in vitro differentiation of Leishmania (Viannia) braziliensis: First evidence of modulation during morphological transition. Biochimie, v.113, p.28-36, 2017. | pt_BR |
ISSN | 0300-9084 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/18731 | |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | restricted access | |
Subject in Portuguese | Cloridato | pt_BR |
Subject in Portuguese | Leishmania braziliensis | pt_BR |
Title | Increasing in cysteine proteinase B expression and enzymatic activity during in vitro differentiation of Leishmania (Viannia) braziliensis: First evidence of modulation during morphological transition | pt_BR |
Type | Article | |
Abstract | Leishmania (Viannia) braziliensis presents adaptive protease-dependent mechanisms, as cysteine proteinases B (CPB). This study investigates the expression of three cpb gene isoforms and CPB enzymatic activity during the parasite differentiation. Relative expression levels of LbrM.08.0810 gene were assessed, exhibiting a higher quantity of transcripts in the logarithmic promastigotes phase than in the stationary promastigotes phase (>1.5 times). The cbp gene tends to decrease during acid pH shock and increases when the temperature rises (>1.3 times). LbrM.08.0820 and LbrM.08.0830 genes exhibited similar expression profiles to LbrM.08.0810 gene, with lower levels being observed overall. The proteolytic activity exhibits a gradual increase during the parasite's differentiation with low levels in samples of logarithmic promastigotes phase (3.2 ± 0.08 mmol min(-1) mg protein(-1)) to a peak of activity after 72 h of incubation at 32 °C (4.2 ± 0.026 mmol min(-1) mg protein(-1)) followed by a subsequent decrease of 68 % of peak activity levels after 96 h of incubation at 32 °C (2.8 ± 0.37 mmol min(-1) mg protein(-1)). These activities were also measured in the presence of selective inhibitors for cysteine proteinases, such as Z-Phe-Phe-fluoromethyl ketone and trans-epoxysuccinyl-L-leucylamido(4-guanidino)butane, demonstrating their source as cathepsin-like proteinases. To the best of our knowledge, this report presents the first description of a modulation of cathepsin L-like expression during the L. (V.) braziliensis in vitro differentiation induced by acid pH and high temperature. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultraestrutura Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultraestrutura Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Leishmania (Viannia) braziliensis | pt_BR |
Subject | Cysteine proteinases B | pt_BR |
Subject | Cathepsin L | pt_BR |
Subject | Z-Phe-Arg 7-amido-4-methylcoumarin | pt_BR |
Subject | hydrochloride | pt_BR |
Subject | Z-Phe-Phe-fluoromethyl ketoneand | pt_BR |
Subject | Trans-epoxysuccinyl-L-leucylamido(4- guanidino)butane | pt_BR |
DeCS | Leishmania braziliensis | pt_BR |
DeCS | Cloridato | pt_BR |
DeCS | Catepsina L | pt_BR |
DeCS | Cisteína Proteases | pt_BR |
e-ISSN | 10.1016/j.biochi.2016.11.015 | |
e-ISSN | 1638-6183 | |
Embargo date | 2030-01-01 | |