| Author | Silva Junior, Haroldo Cid da | |
| Author | Moura Junior, Sérgio da Silva e | |
| Author | Mendonça, Marcos César Lima de | |
| Author | Pereira, Mirian Claudia de Souza | |
| Author | Nogueira, Alanderson da Rocha | |
| Author | Azevedo, Maria Luiza Borges de | |
| Author | Leite, José Paulo Gagliardi | |
| Author | Moraes, Márcia Terezinha Baroni de | |
| Access date | 2017-05-09T17:02:54Z | |
| Available date | 2017-05-09T17:02:54Z | |
| Document date | 2012 | |
| Citation | SILVA JUNIOR, Harold Cid da; etal. Comparison of two eukaryotic systems for the expression of VP6 protein of rotavirus specie A: transient gene expression in HEK293-T cells and insect cell-baculovirus system. Biotechnol Lett, v.34, p.1623–1627, 2012. | pt_BR |
| ISSN | 0141-5492 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/18799 | |
| Language | eng | pt_BR |
| Publisher | Springer Verlag (Germany) | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Rotavirus A | pt_BR |
| Subject in Portuguese | Vacina | pt_BR |
| Subject in Portuguese | Diagnóstico | pt_BR |
| Subject in Portuguese | Sistemas eucarióticos | pt_BR |
| Title | Comparison of two eukaryotic systems for the expression of VP6 protein of rotavirus specie A: transient gene expression in HEK293-T cells and insect cell-baculovirus system | pt_BR |
| Type | Article | |
| DOI | 10.1007/s10529-012-0946-z | |
| Abstract | The VP6 protein of rotavirus A (RVA) is a target antigen used for diagnostic assays and also for the development of new RVA vaccines. We have compared the expression of VP6 protein in human embryonic kidney (HEK293-T) cells with results obtained using a well-established insect cell-baculovirus system. The recombinant VP6 (rVP6) expressed in HEK293-T cells did not present degradation and also retained the ability to form trimers. In the insect cell-baculovirus system, rVP6 was expressed at higher levels and with protein degradation as well as partial loss of ability to form trimers was observed. Therefore, HEK293-T cells represent a less laborious alternative system than insect cells for expression of rVP6 from human RVA. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultraestrutura Celular. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultraestrutura Celular. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Bio-Manguinhos. Laboratório de Tecnologia Recombinante. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Subject | Rotavirus A | pt_BR |
| Subject | VP6 protein | pt_BR |
| Subject | HEK293-T cells | pt_BR |
| Subject | Sf9 cells | pt_BR |
| Subject | Diagnosis | pt_BR |
| Subject | Vaccine | pt_BR |
| Subject | eukaryotic systems | pt_BR |
| e-ISSN | 1573-6776 | |
| Embargo date | 2030-01-01 | |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
