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2030-01-01
Sustainable Development Goals
03 Saúde e Bem-EstarCollections
- IOC - Artigos de Periódicos [12969]
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COMPARISON OF TWO EUKARYOTIC SYSTEMS FOR THE EXPRESSION OF VP6 PROTEIN OF ROTAVIRUS SPECIE A: TRANSIENT GENE EXPRESSION IN HEK293-T CELLS AND INSECT CELL-BACULOVIRUS SYSTEM
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The VP6 protein of rotavirus A (RVA) is a target antigen used for diagnostic assays and also for the development of new RVA vaccines. We have compared the expression of VP6 protein in human embryonic kidney (HEK293-T) cells with results obtained using a well-established insect cell-baculovirus system. The recombinant VP6 (rVP6) expressed in HEK293-T cells did not present degradation and also retained the ability to form trimers. In the insect cell-baculovirus system, rVP6 was expressed at higher levels and with protein degradation as well as partial loss of ability to form trimers was observed. Therefore, HEK293-T cells represent a less laborious alternative system than insect cells for expression of rVP6 from human RVA.
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