Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/18799
Title: Comparison of two eukaryotic systems for the expression of VP6 protein of rotavirus specie A: transient gene expression in HEK293-T cells and insect cell-baculovirus system
Authors: Silva Junior, Haroldo Cid da
Moura Junior, Sérgio da Silva e
Mendonça, Marcos César Lima de
Pereira, Mirian Claudia de Souza
Nogueira, Alanderson da Rocha
Azevedo, Maria Luiza Borges de
Leite, José Paulo Gagliardi
Moraes, Márcia Terezinha Baroni de
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultraestrutura Celular. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultraestrutura Celular. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Bio-Manguinhos. Laboratório de Tecnologia Recombinante. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ. Brasil.
Abstract: The VP6 protein of rotavirus A (RVA) is a target antigen used for diagnostic assays and also for the development of new RVA vaccines. We have compared the expression of VP6 protein in human embryonic kidney (HEK293-T) cells with results obtained using a well-established insect cell-baculovirus system. The recombinant VP6 (rVP6) expressed in HEK293-T cells did not present degradation and also retained the ability to form trimers. In the insect cell-baculovirus system, rVP6 was expressed at higher levels and with protein degradation as well as partial loss of ability to form trimers was observed. Therefore, HEK293-T cells represent a less laborious alternative system than insect cells for expression of rVP6 from human RVA.
Keywords: Rotavirus A
VP6 protein
HEK293-T cells
Sf9 cells
Diagnosis
Vaccine
eukaryotic systems
keywords: Rotavirus A
Vacina
Diagnóstico
Sistemas eucarióticos
Issue Date: 2012
Publisher: Springer Verlag (Germany)
Citation: SILVA JUNIOR, Harold Cid da; etal. Comparison of two eukaryotic systems for the expression of VP6 protein of rotavirus specie A: transient gene expression in HEK293-T cells and insect cell-baculovirus system. Biotechnol Lett, v.34, p.1623–1627, 2012.
DOI: 10.1007/s10529-012-0946-z
ISSN: 0141-5492
Copyright: restricted access
Appears in Collections:IOC - Artigos de Periódicos

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