Title:	Transmission of Campylobacter coli in chicken embryos
Authors:	Rossi, Daise Aparecida Fonseca, Belchiolina Beatriz Melo, Roberta Torres de Felipe, Gutembergue da Silva Silva, Paulo Lourenço da Mendonça, Eliane Pereira Filgueiras, Ana Luzia Lauria Beletti, Marcelo Emilio
Affilliation:	Universidade Federal de Uberlândia. Laboratório de Biotecnologia Animal Aplicada. Uberlândia, MG, Brasil. Universidade Federal de Uberlândia. Laboratório de Biotecnologia Animal Aplicada. Uberlândia, MG, Brasil / Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Uberlândia, MG, Brasil. Universidade Federal de Uberlândia. Laboratório de Biotecnologia Animal Aplicada. Uberlândia, MG, Brasil. Universidade Federal de Uberlândia. Laboratório de Biotecnologia Animal Aplicada. Uberlândia, MG, Brasil. Universidade Federal de Uberlândia. Laboratório de Biotecnologia Animal Aplicada. Uberlândia, MG, Brasil. Universidade Federal de Uberlândia. Laboratório de Biotecnologia Animal Aplicada. Uberlândia, MG, Brasil. Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ. Brasil. Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Uberlândia, MG, Brasil.
Abstract:	Campylobacter coli is an important species involved in human cases of enteritis, and chickens are carriers of the pathogen mainly in developing country. The current study aimed to evaluate the transmission of C. coli and its pathogenic effects in chicken embryos. Breeder hens were inoculated intra-esophageally with C. coli isolated from chickens, and their eggs and embryos were analyzed for the presence of bacteria using realtime PCR and plate culture. The viability of embryos was verified. In parallel, SPF eggs were inoculated with C. coli in the air sac; after incubation, the embryos were submitted to the same analysis as the embryos from breeder hens. In embryos and fertile eggs from breeder hens, the bacterium was only identified by molecular methods; in the SPF eggs, however, the bacterium was detected by both techniques. The results showed no relationship between embryo mortality and positivity for C. coli in the embryos from breeder hens. However, the presence of bacteria is a cause of precocious mortality for SPF embryos. This study revealed that although the vertical transmission is a possible event, the bacteria can not grow in embryonic field samples.
Keywords:	Campylobacter coli Viability Transmission Breeder hens
keywords:	Campylobacter coli Viabilidade Transmissão Galinhas reprodutoras
Issue Date:	2012
Publisher:	Sociedade Brasileira de Microbiologia
Citation:	ROSSI, Daise Aparecida; et al. Transmission of Campylobacter coli in chicken embryos. Brazilian Journal of Microbiology , p.535-543, 2012.
ISSN:	1517-8382
Copyright:	open access
Appears in Collections:	IOC - Artigos de Periódicos

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