Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/18831
Title: Heavy metal tolerance (Cr, Ag and Hg) in bacteria isolated from sewage
Authors: Silva, Agostinho A. de Lima e
Carvalho, Márcia A. Ribeiro de
Souza, Sérgio A. L de
Dias, Patrícia M. Teixeira
Silva Filho, Renato G. da
Saramago, Carmen S. de Meirelles
Bento, Cleonice A. de Melo
Hofer, Ernesto
Affilliation: Universidade Federal do Estado do Rio de Janeiro. Departamento de Microbiologia e Parasitologia. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Estado do Rio de Janeiro. Departamento de Microbiologia e Parasitologia. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Estado do Rio de Janeiro. Departamento de Microbiologia e Parasitologia. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Estado do Rio de Janeiro. Departamento de Microbiologia e Parasitologia. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Estado do Rio de Janeiro. Departamento de Microbiologia e Parasitologia. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Estado do Rio de Janeiro. Departamento de Microbiologia e Parasitologia. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Estado do Rio de Janeiro. Departamento de Microbiologia e Parasitologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ. Brasil.
Abstract: Samples of sewage from a university hospital and a chemistry technical school were analysed for the percentage of bacterial tolerance to chromium (Cr), silver (Ag) and mercury (Hg). Additionally, we investigated the effect of these metals on pigmentation and on some enzymatic activities of the metal tolerant strains isolated, as well as antimicrobial resistance in some metal tolerant Enterobacteriaceae strains. Tolerance to Cr was observed mainly in Gram positive bacteria while in the case of Ag and Hg the tolerant bacteria were predominately Gram negative. Hg was the metal for which the percentage of tolerance was significantly higher, especially in samples from the hospital sewage (4.1%). Mercury also had the most discernible effect on color of the colonies. Considering the effect of metals on the respiratory enzymes, one strain of Ag-tolerant Bacillus sp. and one of Hg-tolerant P. aeruginosa were unable to produce oxidase in the presence of Ag and Hg, respectively, while the expression of gelatinase was largely inhibited in various Gram negative strains (66% by Cr). Drug resistance in Hg-tolerant Enterobacteriaceae strains isolated from the university hospital sewage was greater than 80%, with prevalence of multiple resistance, while the Ag-tolerant strains from the same source showed about 34% of resistance, with the predominance of mono-resistance. Our results showed that, despite the ability of metal tolerant strains to survive and grow in the presence of these elements, the interactions with these metals may result in metabolic or phisiological changes in this group of bacteria.
Keywords: metal tolerance
sewage
pigment
enzymatic inhibition
antimicrobial resistance
keywords: Tolerância a metal
Resistência antimicrobiana
Pigmento
Inibição enzimática
Esgoto
Issue Date: 2012
Publisher: Sociedade Brasileira de Microbiologia
Citation: SILVA, Agostinho A. de Lima e; et al. Heavy metal tolerance (Cr, Ag and Hg) in bacteria isolated from sewage. Brazilian Journal of Microbiology, p.1620-1631, 2012.
ISSN: 1517-8382
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

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