| Author | Miranda, Íngara Keisle São Paulo Barretto | |
| Author | Miranda, Anderson Fontes Suzart | |
| Author | Souza, Fernanda Vidigal Duarte | |
| Author | Santos, Marcos André Vannier dos | |
| Author | Pirovani, Carlos Priminho | |
| Author | Pepe, Iuri Muniz | |
| Author | Rodowanski, Ivanoé João | |
| Author | Ferreira, Katiúcia Tícila de Souza Eduvirgens | |
| Author | Vaz, Luciano Mendes Souza | |
| Author | Assis, Sandra Aparecida de | |
| Access date | 2017-05-12T17:34:18Z | |
| Available date | 2017-05-12T17:34:18Z | |
| Document date | 2017 | |
| Citation | MIRANDA, I. K. S. P. B. et al. The biochemical characterization, stabilization studies and the antiproliferative effect of bromelain against B16F10 murine melanoma cells. International Journal of Food Sciences and Nutrition, 2016. | pt_BR |
| ISSN | 0963-7486 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/18838 | |
| Sponsorship | Fundação de Amparo a Pesquisa do Estado da Bahia (FAPESB), Coordenação de Aperfeiçoamento Pessoal de Nível Superior (CAPES), CNPq and the Post Graduate Program in Plant Genetic Resources – UEFS | pt_BR |
| Language | eng | pt_BR |
| Publisher | Taylor & Francis | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Melanoma | pt_BR |
| Subject in Portuguese | Estabilidade enzimática | pt_BR |
| Subject in Portuguese | Bromelaina | pt_BR |
| Subject in Portuguese | Metodologia | pt_BR |
| Subject in Portuguese | Células tumorais | pt_BR |
| Subject in Portuguese | Ananas | pt_BR |
| Title | The biochemical characterization, stabilization studies and the antiproliferative effect of bromelain against B16F10 murine melanoma cells | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1080/09637486.2016.1254599 | |
| Abstract | The current study aims to extract bromelain from different parts (stem, crown, peels, pulp and leaves) of Ananas comosus var. comosus AGB 772; to determine of optimum pH and temperature; to test bromelain stability in disodium EDTA and sodium benzoate, and to investigate its pharmacological activity on B16F10 murine melanoma cells in vitro. The highest enzymatic activity was found in bromelain extracted from the pulp and peel. The optimum bromelain pH among all studied pineapple parts was 6.0. The optimum temperature was above 50 °C in all bromelain extracts. The fluorescence analysis confirmed the stability of bromelain in the presence of EDTA and sodium benzoate. Bromelain was pharmacologically active against B16F10 melanoma cells and it was possible verifying approximately 100% inhibition of tumor cell proliferation in vitro. Since bromelain activity was found in different parts of pineapple plants, pineapple residues from the food industry may be used for bromelain extraction. | pt_BR |
| Affilliation | State University of Feira de Santana. Health Department. Feira de Santana, BA, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Parasitic Biology Laboratory. Salvador, BA, Brasil | pt_BR |
| Affilliation | Embrapa Mandioca e Fruticultura. Cruz das Almas, BA, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Parasitic Biology Laboratory. Salvador, BA, Brasil | pt_BR |
| Affilliation | State University of Santa Cruz. Biological Sciences Department Biotechnology and Genetics Center. llhéus, BA, Brazil | pt_BR |
| Affilliation | Federal University of Bahia. Laboratory of Optical Properties. Physics Department. Salvador, BA, Brazil | pt_BR |
| Affilliation | Federal University of Bahia. Laboratory of Optical Properties. Physics Department. Salvador, BA, Brazil | pt_BR |
| Affilliation | State University of Santa Cruz. Biological Sciences Department Biotechnology and Genetics Center. llhéus, BA, Brazil | pt_BR |
| Affilliation | State University of Feira de Santana. Technology, Sanitation, Hydric Resources and Environment Department. Feira de Santana, BA, Brazil | pt_BR |
| Affilliation | State University of Feira de Santana. Health Department. Feira de Santana, BA, Brazil | pt_BR |
| Subject | Antiproliferative effect in vitro | pt_BR |
| Subject | Murine melanomacells | pt_BR |
| Subject | Cell culture | pt_BR |
| Subject | Enzyme stability | pt_BR |
| Subject | Bromelainas | pt_BR |
| Subject | Response surface methodology | pt_BR |
| DeCS | Melanoma | pt_BR |
| DeCS | Estabilidade enzimática | pt_BR |
| DeCS | Bromelainas | pt_BR |
| DeCS | Metodologia | pt_BR |
| DeCS | Ananas | pt_BR |
