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THE BIOCHEMICAL CHARACTERIZATION, STABILIZATION STUDIES AND THE ANTIPROLIFERATIVE EFFECT OF BROMELAIN AGAINST B16F10 MURINE MELANOMA CELLS
Miranda, Íngara Keisle São Paulo Barretto et al. | Date Issued: 2017
Author
Miranda, Íngara Keisle São Paulo Barretto
Miranda, Anderson Fontes Suzart
Souza, Fernanda Vidigal Duarte
Santos, Marcos André Vannier dos
Pirovani, Carlos Priminho
Pepe, Iuri Muniz
Rodowanski, Ivanoé João
Ferreira, Katiúcia Tícila de Souza Eduvirgens
Vaz, Luciano Mendes Souza
Assis, Sandra Aparecida de
Affilliation
State University of Feira de Santana. Health Department. Feira de Santana, BA, Brazil
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Parasitic Biology Laboratory. Salvador, BA, Brasil
Embrapa Mandioca e Fruticultura. Cruz das Almas, BA, Brazil
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Parasitic Biology Laboratory. Salvador, BA, Brasil
State University of Santa Cruz. Biological Sciences Department Biotechnology and Genetics Center. llhéus, BA, Brazil
Federal University of Bahia. Laboratory of Optical Properties. Physics Department. Salvador, BA, Brazil
Federal University of Bahia. Laboratory of Optical Properties. Physics Department. Salvador, BA, Brazil
State University of Santa Cruz. Biological Sciences Department Biotechnology and Genetics Center. llhéus, BA, Brazil
State University of Feira de Santana. Technology, Sanitation, Hydric Resources and Environment Department. Feira de Santana, BA, Brazil
State University of Feira de Santana. Health Department. Feira de Santana, BA, Brazil
Abstract
The current study aims to extract bromelain from different parts (stem, crown, peels, pulp and leaves) of Ananas comosus var. comosus AGB 772; to determine of optimum pH and temperature; to test bromelain stability in disodium EDTA and sodium benzoate, and to investigate its pharmacological activity on B16F10 murine melanoma cells in vitro. The highest enzymatic activity was found in bromelain extracted from the pulp and peel. The optimum bromelain pH among all studied pineapple parts was 6.0. The optimum temperature was above 50 °C in all bromelain extracts. The fluorescence analysis confirmed the stability of bromelain in the presence of EDTA and sodium benzoate. Bromelain was pharmacologically active against B16F10 melanoma cells and it was possible verifying approximately 100% inhibition of tumor cell proliferation in vitro. Since bromelain activity was found in different parts of pineapple plants, pineapple residues from the food industry may be used for bromelain extraction.
Keywords in Portuguese
Melanoma
Estabilidade enzimática
Bromelaina
Metodologia
Células tumorais
Ananas
 
Keywords
Antiproliferative effect in vitro
Murine melanomacells
Cell culture
Enzyme stability
Bromelainas
Response surface methodology
 
DeCS
Melanoma
Estabilidade enzimática
Bromelainas
Metodologia
Ananas
 
Publisher
Taylor & Francis
Citation
MIRANDA, I. K. S. P. B. et al. The biochemical characterization, stabilization studies and the antiproliferative effect of bromelain against B16F10 murine melanoma cells. International Journal of Food Sciences and Nutrition, 2016.
DOI
10.1080/09637486.2016.1254599
ISSN
0963-7486