Author | Costa, Giselle Calasans de Souza | |
Author | Azevedo, Rochele | |
Author | Gadelha, Sandra Rocha | |
Author | Kashima, Simone Haddad | |
Author | Muricy, Gabriel | |
Author | Gallazzi, Viviana Nilla Olavarria | |
Author | Covas, Dimas Tadeu | |
Author | Takayanagui, Osvaldo Massaiti | |
Author | Castro Filho, Bernardo Galvão | |
Author | Alcantara, Luiz Carlos Júnior | |
Access date | 2011-04-12T23:14:32Z | |
Available date | 2011-04-12T23:14:32Z | |
Document date | 2009 | |
Citation | COSTA, G. C. S. et al. Polymorphisms at GLUT1 gene are not associated with the development of TSP/HAM in Brazilian HTLV-1 infected individuals and the discovery of a new polymorphism at GLUT1 gene. Journal of Medical Virology, v. 81, n. 3, p. 552-557, 2009. | pt_BR |
ISSN | 0146-6615 | |
URI | https://www.arca.fiocruz.br/handle/icict/1892 | |
Language | eng | pt_BR |
Rights | open access | |
Title | Polymorphisms at GLUT1 gene are not associated with the development of TSP/HAM in Brazilian HTLV-1 infected individuals and the discovery of a new polymorphism at GLUT1 gene | pt_BR |
Type | Article | pt_BR |
DOI | 10.1002/jmv.21421 | |
Abstract | The development of HTLV-1 associated clinical manifestations, such as TSP/HAM and ATLL, occur in 2-4% of the infected population and it is still unclear why this infection remains asymptomatic in most infected carriers. Recently, it has been demonstrated that HTLV uses the Glucose transporter type 1 (GLUT1) to infect T-CD4(+) lymphocytes and that single nucleotide polymorphisms (SNP) in the GLUT1 gene are associated with diabetic nephropathy in patients with diabetes mellitus in different populations. These polymorphisms could contribute to a higher GLUT1 protein expression on cellular membrane, facilitating the entry of HTLV and its transmission cell by cell. This could result in a higher provirus load and consequently in the development of TSP/HAM. To evaluate the role of GLUT1 gene polymorphisms in the development of TSP/HAM in HTLV-1 infected individuals, the g.22999G > T, g.15339T > C and c.-2841A > T sites were analyzed by PCR/RFLP or sequencing in 244 infected individuals and 102 normal controls. The proviral load of the HTLV-1 infected patients was also analyzed using Real Time Quantitative PCR. Genotypic and allelic frequencies of the three sites did not differ significantly between controls and HTLV-1 infected individuals. There was no difference in genotypic and allelic distributions among patients as to the presence or absence of HTLV-1 associated clinic manifestations. As regards the quantification of the provirus load, we observed a significant reduction in the asymptomatic individuals compared with the oligosymptomatic and TSP/HAM individuals. These results suggest that g.22999G > T, g.15339T > C, and c.-2841A > T SNP do not contribute to HTLV-1 infection nor to the genetic susceptibility of TSP/HAM in Brazilian HTLV-1 infected individuals. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil | pt_BR |
Affilliation | Hemocentro de Ribeirão Preto. São Paulo, SP, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil | pt_BR |
Affilliation | Hemocentro de Ribeirão Preto. São Paulo, SP, Brasil | pt_BR |
Affilliation | Fundação Bahiana para o Desenvolvimento das Ciências. Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil | pt_BR |
Affilliation | Hemocentro de Ribeirão Preto. São Paulo, SP, Brasil | pt_BR |
Affilliation | Universidade de São Faculdade de Medicina de Ribeirão Preto. Psiquiatria e Psicologia Médica. Departamento de Neurologia. São Paulo, SP, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil | pt_BR |
Subject | HTLV-1 | pt_BR |
Subject | GLUT1 | pt_BR |
Subject | Polymorphisms | pt_BR |
DeCS | Transportador de Glucose Tipo 1 | pt_BR |
DeCS | Infecções por HTLV-I | pt_BR |
DeCS | Virus Linfotrópico de Células T Humanas Tipo 1 | pt_BR |
DeCS | Paraparesia Espástica Tropical | pt_BR |
DeCS | Polimorfismo Genético | pt_BR |