Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/1892
Title: Polymorphisms at GLUT1 gene are not associated with the development of TSP/HAM in Brazilian HTLV-1 infected individuals and the discovery of a new polymorphism at GLUT1 gene
Authors: Costa, Giselle Calasans de Souza
Azevedo, Rochele
Gadelha, Sandra Rocha
Kashima, Simone Haddad
Muricy, Gabriel
Gallazzi, Viviana Nilla Olavarria
Covas, Dimas Tadeu
Takayanagui, Osvaldo Massaiti
Castro Filho, Bernardo Galvão
Alcantara, Luiz Carlos Júnior
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil
Hemocentro de Ribeirão Preto. São Paulo, SP, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil
Hemocentro de Ribeirão Preto. São Paulo, SP, Brasil
Fundação Bahiana para o Desenvolvimento das Ciências. Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil
Hemocentro de Ribeirão Preto. São Paulo, SP, Brasil
Universidade de São Faculdade de Medicina de Ribeirão Preto. Psiquiatria e Psicologia Médica. Departamento de Neurologia. São Paulo, SP, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil
Abstract: The development of HTLV-1 associated clinical manifestations, such as TSP/HAM and ATLL, occur in 2-4% of the infected population and it is still unclear why this infection remains asymptomatic in most infected carriers. Recently, it has been demonstrated that HTLV uses the Glucose transporter type 1 (GLUT1) to infect T-CD4(+) lymphocytes and that single nucleotide polymorphisms (SNP) in the GLUT1 gene are associated with diabetic nephropathy in patients with diabetes mellitus in different populations. These polymorphisms could contribute to a higher GLUT1 protein expression on cellular membrane, facilitating the entry of HTLV and its transmission cell by cell. This could result in a higher provirus load and consequently in the development of TSP/HAM. To evaluate the role of GLUT1 gene polymorphisms in the development of TSP/HAM in HTLV-1 infected individuals, the g.22999G > T, g.15339T > C and c.-2841A > T sites were analyzed by PCR/RFLP or sequencing in 244 infected individuals and 102 normal controls. The proviral load of the HTLV-1 infected patients was also analyzed using Real Time Quantitative PCR. Genotypic and allelic frequencies of the three sites did not differ significantly between controls and HTLV-1 infected individuals. There was no difference in genotypic and allelic distributions among patients as to the presence or absence of HTLV-1 associated clinic manifestations. As regards the quantification of the provirus load, we observed a significant reduction in the asymptomatic individuals compared with the oligosymptomatic and TSP/HAM individuals. These results suggest that g.22999G > T, g.15339T > C, and c.-2841A > T SNP do not contribute to HTLV-1 infection nor to the genetic susceptibility of TSP/HAM in Brazilian HTLV-1 infected individuals.
Keywords: HTLV-1
GLUT1
Polymorphisms
DeCS: Transportador de Glucose Tipo 1
Infecções por HTLV-I
Virus Linfotrópico de Células T Humanas Tipo 1
Paraparesia Espástica Tropical
Polimorfismo Genético
Issue Date: 2009
Citation: COSTA, G. C. S. et al. Polymorphisms at GLUT1 gene are not associated with the development of TSP/HAM in Brazilian HTLV-1 infected individuals and the discovery of a new polymorphism at GLUT1 gene. Journal of Medical Virology, v. 81, n. 3, p. 552-557, 2009.
DOI: 10.1002/jmv.21421
ISSN: 0146-6615
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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