Author | Oliveira, I. C | |
Author | Almeida, R. C. C. | |
Author | Hofer, Ernesto | |
Author | Almeida, P. F. | |
Access date | 2017-05-18T12:32:58Z | |
Available date | 2017-05-18T12:32:58Z | |
Document date | 2012 | |
Citation | OLIVEIRA, I. C. et al. Bacteriophage amplification assay for detection of Listeria Spp. using virucidal laser treatment. Brazilian Journal of Microbiology, p. 1128-1136, 2012. | pt_BR |
ISSN | 1517-8382 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/18962 | |
Language | eng | pt_BR |
Publisher | Sociedade Brasileira de Microbiologia | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Listeria | pt_BR |
Subject in Portuguese | Bacteriófago A511 | pt_BR |
Subject in Portuguese | Luz laser | pt_BR |
Subject in Portuguese | Detecção | pt_BR |
Subject in Portuguese | Leite desnatado | pt_BR |
Title | Bacteriophage amplification assay for detection of Listeria Spp. using virucidal laser treatment | pt_BR |
Type | Article | pt_BR |
Abstract | A protocol for the bacteriophage amplification technique was developed for quantitative detection of viable
Listeria monocytogenes cells using the A511 listeriophage with plaque formation as the end-point assay.
Laser and toluidine blue O (TBO) were employed as selective virucidal treatment for destruction of
exogenous bacteriophage. Laser and TBO can bring a total reduction in titer phage (ca. 108 pfu/mL) without
affecting the viability of L. monocytogenes cells. Artificially inoculated skimmed milk revealed mean
populations of the bacteria as low as between 13 cfu/mL (1.11 log cfu/mL), after a 10-h assay duration.
Virucidal laser treatment demonstrated better protection of Listeria cells than the other agents previously
tested. The protocol was faster and easier to perform than standard procedures. This protocol constitutes an
alternative for rapid, sensitive and quantitative detection of L. monocytogenes. | pt_BR |
Affilliation | Universidade Estadual da Bahia. Departamento de Ciências da Vida. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade Estadual da Bahia. Escola de Nutrição. Departamento de Ciência dos Alimentos. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bacteriologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Estadual da Bahia. Instituto de Ciências da Saúde. Departamento de Ciências da Biointeração. Salvador, BA, Brasil. | pt_BR |
Subject | Listeria | pt_BR |
Subject | bacteriophage A511 | pt_BR |
Subject | laser light | pt_BR |
Subject | detection | pt_BR |
Subject | skimmed milk | pt_BR |
e-ISSN | 1678-4405 | |