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Title: Bacteriophage amplification assay for detection of Listeria Spp. using virucidal laser treatment
Authors: Oliveira, I. C
Almeida, R. C. C.
Hofer, Ernesto
Almeida, P. F.
Affilliation: Universidade Estadual da Bahia. Departamento de Ciências da Vida. Salvador, BA, Brasil
Universidade Estadual da Bahia. Escola de Nutrição. Departamento de Ciência dos Alimentos. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bacteriologia. Rio de Janeiro, RJ, Brasil.
Universidade Estadual da Bahia. Instituto de Ciências da Saúde. Departamento de Ciências da Biointeração. Salvador, BA, Brasil.
Abstract: A protocol for the bacteriophage amplification technique was developed for quantitative detection of viable Listeria monocytogenes cells using the A511 listeriophage with plaque formation as the end-point assay. Laser and toluidine blue O (TBO) were employed as selective virucidal treatment for destruction of exogenous bacteriophage. Laser and TBO can bring a total reduction in titer phage (ca. 108 pfu/mL) without affecting the viability of L. monocytogenes cells. Artificially inoculated skimmed milk revealed mean populations of the bacteria as low as between 13 cfu/mL (1.11 log cfu/mL), after a 10-h assay duration. Virucidal laser treatment demonstrated better protection of Listeria cells than the other agents previously tested. The protocol was faster and easier to perform than standard procedures. This protocol constitutes an alternative for rapid, sensitive and quantitative detection of L. monocytogenes.
Keywords: Listeria
bacteriophage A511
laser light
skimmed milk
keywords: Listeria
Bacteriófago A511
Luz laser
Leite desnatado
Issue Date: 2012
Publisher: Sociedade Brasileira de Microbiologia
Citation: OLIVEIRA, I. C. et al. Bacteriophage amplification assay for detection of Listeria Spp. using virucidal laser treatment. Brazilian Journal of Microbiology, p. 1128-1136, 2012.
ISSN: 1517-8382
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

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