| Author | Castro, Daniele P. | |
| Author | Moraes, Caroline S. | |
| Author | Gonzalez, Marcelo S. | |
| Author | Ribeiro, Ivone M. | |
| Author | Tomassini, Therezinha C. B. | |
| Author | Azambuja, Patricia | |
| Author | Garcia, Eloi S. | |
| Access date | 2017-05-25T17:46:01Z | |
| Available date | 2017-05-25T17:46:01Z | |
| Document date | 2012 | |
| Citation | CASTRO, Daniele P. et al. Physalin B inhibits Trypanosoma cruzi infection in the gut of Rhodnius prolixus by affecting the immune system and microbiota. Journal of Insect Physiology, v.58, p.1620–1625, Oct. 2012. | pt_BR |
| ISSN | 0022-1910 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/19011 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Rhodnius prolixus | pt_BR |
| Subject in Portuguese | Trypanosoma cruzi | pt_BR |
| Subject in Portuguese | Trato Gastrointestinal | pt_BR |
| Subject in Portuguese | Infecção | pt_BR |
| Subject in Portuguese | Microbiota | pt_BR |
| Subject in Portuguese | Óxido Nítrico | pt_BR |
| Subject in Portuguese | Peptídeo antimicrobiano | pt_BR |
| Title | Physalin B inhibits Trypanosoma cruzi infection in the gut of Rhodnius prolixus by affecting the immune system and microbiota | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.jinsphys.2012.10.001 | |
| Abstract | Physalin B is a natural secosteroidal, extracted from the Solanaceae plant, Physalis angulata, and it presents immune-modulator effects on the bloodsucking bug, Rhodnius prolixus. In this work, R. prolixus was treated with physalin B at a concentration of 1 mg/ml of blood meal (oral application), or 20 ng/insect (applied topically) or 57 ng/cm(2) of filter paper (contact treatment), and infected with Trypanosoma cruzi Dm28c clone (2×10(6) epimastigotes/insect). The three types of applications significantly decreased the number of T. cruzi Dm28c in the gut comparing with the non-treated infected insects (controls). All groups of infected insects treated with physalin B had higher numbers of bacterial microbiota in the gut than the non-treated controls infected with T. cruzi. We observed that the infected physalin B insects with topical and contact treatments had a lower antibacterial activity in the gut when compared with control infected insects. Furthermore, infected insects with the physalin B oral treatment produced higher levels of nitrite and nitrate in the gut than control infected insects. These results demonstrate that physalin B decreases the T. cruzi transmission by inhibiting the parasite development in the insect vector R. prolixus. Herein the importance of physalin B modulation on the immune system and microbiota population in terms of parasite development and transmission are discussed. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Universidade Federal Fluminense. Instituo de Biologia. Departamento de Biologia Geral. Laboratório de Biologia de Insetos. Niterói, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Far-Manguinhos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Far-Manguinhos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ. Brasil / Instituto Nacional de Entomologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ. Brasil / Instituto Nacional de Entomologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Rhodnius prolixus | pt_BR |
| Subject | Physalis angulata L | pt_BR |
| Subject | Physalin | pt_BR |
| Subject | Trypanosoma cruzi | pt_BR |
| Subject | Gut infection | pt_BR |
| Subject | Antimicrobial peptide | pt_BR |
| Subject | Nitric oxide | pt_BR |
| Subject | Microbiota | pt_BR |
| e-ISSN | 1879-1611 | |
| Embargo date | 2030-01-01 | |
