Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/19011
Title: Physalin B inhibits Trypanosoma cruzi infection in the gut of Rhodnius prolixus by affecting the immune system and microbiota
Authors: Castro, Daniele P.
Moraes, Caroline S.
Gonzalez, Marcelo S.
Ribeiro, Ivone M.
Tomassini, Therezinha C. B.
Azambuja, Patrícia
Garcia, Eloi S.
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ. Brasil.
Universidade Federal Fluminense. Instituo de Biologia. Departamento de Biologia Geral. Laboratório de Biologia de Insetos. Niterói, RJ, Brasil.
Fundação Oswaldo Cruz. Far-Manguinhos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Far-Manguinhos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ. Brasil / Instituto Nacional de Entomologia Molecular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ. Brasil / Instituto Nacional de Entomologia Molecular. Rio de Janeiro, RJ, Brasil.
Abstract: Physalin B is a natural secosteroidal, extracted from the Solanaceae plant, Physalis angulata, and it presents immune-modulator effects on the bloodsucking bug, Rhodnius prolixus. In this work, R. prolixus was treated with physalin B at a concentration of 1 mg/ml of blood meal (oral application), or 20 ng/insect (applied topically) or 57 ng/cm(2) of filter paper (contact treatment), and infected with Trypanosoma cruzi Dm28c clone (2×10(6) epimastigotes/insect). The three types of applications significantly decreased the number of T. cruzi Dm28c in the gut comparing with the non-treated infected insects (controls). All groups of infected insects treated with physalin B had higher numbers of bacterial microbiota in the gut than the non-treated controls infected with T. cruzi. We observed that the infected physalin B insects with topical and contact treatments had a lower antibacterial activity in the gut when compared with control infected insects. Furthermore, infected insects with the physalin B oral treatment produced higher levels of nitrite and nitrate in the gut than control infected insects. These results demonstrate that physalin B decreases the T. cruzi transmission by inhibiting the parasite development in the insect vector R. prolixus. Herein the importance of physalin B modulation on the immune system and microbiota population in terms of parasite development and transmission are discussed.
Keywords: Rhodnius prolixus
Physalis angulata L
Physalin
Trypanosoma cruzi
Gut infection
Antimicrobial peptide
Nitric oxide
Microbiota
keywords: Rhodnius prolixus
Trypanosoma cruzi
Trato Gastrointestinal
Infecção
Microbiota
Óxido Nítrico
Peptídeo antimicrobiano
Issue Date: 2012
Publisher: Elsevier
Citation: CASTRO, Daniele P. et al. Physalin B inhibits Trypanosoma cruzi infection in the gut of Rhodnius prolixus by affecting the immune system and microbiota. Journal of Insect Physiology, v.58, p.1620–1625, Oct. 2012.
DOI: 10.1016/j.jinsphys.2012.10.001
ISSN: 0022-1910
Copyright: restricted access
Appears in Collections:IOC - Artigos de Periódicos

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