Author | Chagas, Thiago Pavoni Gomes | |
Author | Alves, Ronaldo Mendes | |
Author | Vallim, Deyse Christina | |
Author | Seki, Liliane Miyuki | |
Author | Campos, Leila Carvalho | |
Author | Asensi, Marise Dutra | |
Access date | 2017-05-31T18:49:22Z | |
Available date | 2017-05-31T18:49:22Z | |
Document date | 2011 | |
Citation | CHAGAS, T. P. G. et al. Diversity of genotypes in CTX-M-producing Klebsiella pneumoniae isolated in different hospitals in Brazil. Brazilian Journal of Infectious Diseases, v. 15, n. 5, p. 420-425, 2011. | pt_BR |
ISSN | 1413-8670 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/19078 | |
Sponsorship | FAPERJ e CNPq. | pt_BR |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Klebsiella pneumoniae | pt_BR |
Subject in Portuguese | Β-lactamases | pt_BR |
Subject in Portuguese | Genótipo | pt_BR |
Title | Diversity of genotypes in CTX-M-producing Klebsiella pneumoniae isolated in different hospitals in Brazil | pt_BR |
Type | Article | pt_BR |
Abstract | Objective: The present study was undertaken to characterize CTX-M ESBL-producing Klebsiella
pneumoniae collected from hospitals in different cities of Brazil. Material and Methods: Eighty-five
K. pneumoniae strains isolated from hospitalized patients in six different hospitals of three cities
of Brazil were analyzed. ESBL production was confirmed by the standard double-disk synergy test
and the Etest®. The MIC50 and MIC90 for ESBL-producing isolates were determined by the Etest®
method. The antimicrobial susceptibilities of bacterial isolates were determined using the agar diffusion
method according to the CLSI. Screening for blaTEM, blaSHV, blaCTX-M genes and class 1 integron
was performed by PCR amplification. To determine the genomic diversity of CTX-M-producers,
isolates were analyzed by macrorestriction profile analysis following PFGE. Results and Discussion:
Seventy-one K. pneumoniae isolates were ESBL-producing. PCR and sequencing experiments
detected 38 CTX-M-producing K. pneumoniae belonged to groups CTX-M 1, CTX-M 2, CTX-M 8
and CTX-M 9. The association of different types ESBL (CTX-M, SHV and TEM) was frequent. All
K. pneumoniae isolates carried class 1 integron. PFGE analysis revealed thirty-one clonal types
among CTX-M-producing isolates. The data presented herein illustrate the diversity of genotypes of
CTX-M producing K. pneumoniae among Brazilians hospitals. | pt_BR |
Affilliation | Universidade do Estado do Rio de Janeiro. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil | pt_BR |
Affilliation | Universidade Rural do Rio de Janeiro. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Hospital Infection Research Laboratory. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil | pt_BR |
Subject | Klebsiella pneumoniae | pt_BR |
Subject | β-lactamases | pt_BR |
Subject | Genotype | pt_BR |
DeCS | Klebsiella pneumoniae | pt_BR |
DeCS | Beta lactamases | pt_BR |
DeCS | Genótipo | pt_BR |