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https://www.arca.fiocruz.br/handle/icict/19078
DIVERSITY OF GENOTYPES IN CTX-M-PRODUCING KLEBSIELLA PNEUMONIAE ISOLATED IN DIFFERENT HOSPITALS IN BRAZIL
Author
Affilliation
Universidade do Estado do Rio de Janeiro. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil
Universidade Federal do Rio de Janeiro. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil
Universidade Rural do Rio de Janeiro. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Hospital Infection Research Laboratory. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil
Universidade Federal do Rio de Janeiro. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil
Universidade Rural do Rio de Janeiro. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Hospital Infection Research Laboratory. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil
Abstract
Objective: The present study was undertaken to characterize CTX-M ESBL-producing Klebsiella
pneumoniae collected from hospitals in different cities of Brazil. Material and Methods: Eighty-five
K. pneumoniae strains isolated from hospitalized patients in six different hospitals of three cities
of Brazil were analyzed. ESBL production was confirmed by the standard double-disk synergy test
and the Etest®. The MIC50 and MIC90 for ESBL-producing isolates were determined by the Etest®
method. The antimicrobial susceptibilities of bacterial isolates were determined using the agar diffusion
method according to the CLSI. Screening for blaTEM, blaSHV, blaCTX-M genes and class 1 integron
was performed by PCR amplification. To determine the genomic diversity of CTX-M-producers,
isolates were analyzed by macrorestriction profile analysis following PFGE. Results and Discussion:
Seventy-one K. pneumoniae isolates were ESBL-producing. PCR and sequencing experiments
detected 38 CTX-M-producing K. pneumoniae belonged to groups CTX-M 1, CTX-M 2, CTX-M 8
and CTX-M 9. The association of different types ESBL (CTX-M, SHV and TEM) was frequent. All
K. pneumoniae isolates carried class 1 integron. PFGE analysis revealed thirty-one clonal types
among CTX-M-producing isolates. The data presented herein illustrate the diversity of genotypes of
CTX-M producing K. pneumoniae among Brazilians hospitals.
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