Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/19302
Title: Performance Assessment of Four Chimeric Trypanosoma cruzi Antigens Based on Antigen-Antibody Detection for Diagnosis of Chronic Chagas Disease
Authors: Santos, Fred Luciano Neves
Celedon, Paola Alejandra Fiorani
Zanchin, Nilson Ivo Tonin
Brasil, Tatiana de Arruda Campos
Foti, Leonardo
Souza, Wayner Vieira de
Silva, Edmilson Domingos
Gomes, Yara de Miranda
Krieger, Marco Aurélio
Affilliation: Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.
Molecular Biology Institute of Paraná. Curitiba, PR, Brazil.
Molecular Biology Institute of Paraná. Curitiba, PR, Brazil / Fundação Oswaldo Cruz. Carlos Chagas Institute. Curitiba, PR, Brazil.
Fundação Oswaldo Cruz. Carlos Chagas Institute. Curitiba, PR, Brazil.
Molecular Biology Institute of Paraná. Curitiba, PR, Brazil / Fundação Oswaldo Cruz. Carlos Chagas Institute. Curitiba, PR, Brazil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Biomanguinhos. Rio de Janeiro, RJ, Brazil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.
Molecular Biology Institute of Paraná. Curitiba, PR, Brazil / Fundação Oswaldo Cruz. Carlos Chagas Institute. Curitiba, PR, Brazil.
Abstract: The performance of serologic tests in chronic Chagas disease diagnosis largely depends on the type and quality of the antigen preparations that are used for detection of anti-Trypanosoma cruzi antibodies. Whole-cell T. cruzi extracts or recombinant proteins have shown variation in the performance and cross-reactivity. Synthetic chimeric proteins comprising fragments of repetitive amino acids of several different proteins have been shown to improve assay performances to detect Chagasic infections. Here, we describe the production of four chimeric T. cruzi proteins and the assessment of their performance for diagnostic purposes. Circular Dichroism spectra indicated the absence of well-defined secondary structures, while polydispersity evaluated by Dynamic Light Scattering revealed only minor aggregates in 50 mM carbonate-bicarbonate (pH 9.6), demonstrating that it is an appropriate buffering system for sensitizing microplates. Serum samples from T. cruzi-infected and non-infected individuals were used to assess the performance of these antigens for detecting antibodies against T. cruzi, using both enzyme-linked immunosorbent assay and a liquid bead array platform. Performance parameters (AUC, sensitivity, specificity, accuracy and J index) showed high diagnostic accuracy for all chimeric proteins for detection of specific anti-T. cruzi antibodies and differentiated seropositive individuals from those who were seronegative. Our data suggest that these four chimeric proteins are eligible for phase II studies.
keywords: Trypanosoma cruzi
Doença de Chagas
Testes Sorológicos
Diagnóstico
DeCS: Anticorpos, protozoários / imunologia
Antígenos , protozoários / imunologia
Doença de Chagas / diagnóstico
Ensaio de Imunoadsorção Enzimática / métodos
Trypanosoma cruzi / imunologia
Issue Date: 2016
Citation: SANTOS, F. L. N. et al. Performance Assessment of Four Chimeric Trypanosoma cruzi Antigens Based on Antigen-Antibody Detection for Diagnosis of Chronic Chagas Disease. PLOS ONE, v. 11, n. 8, p. e0161100, 12 ago. 2016.
DOI: 10.1371/journal.pone.0161100
ISSN: 1932-6203
Copyright: open access
Appears in Collections:Biomanguinhos - Artigos de Periódicos
PE - IAM - Artigos de Periódicos

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