Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/1933
Title: Characterization of Shigella spp. by antimicrobial resistance and PCR detection of ipa genes in an infantile population from Porto Velho (Western Amazon region), Brazil
Authors: Silva, Tatiane
Nogueira, Paulo Afonso
Magalhães, Gleiciene Félix
Grava, Andréa Fagundes
Silva, Luiz Hildebrando Pereira da
Orlandi, Patricia Puccinelli
Affilliation: Centro de Pesquisa em Medicina Tropical. Porto Velho, RO, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisa Leônidas e Maria Deane. Manaus. AM, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisa Leônidas e Maria Deane. Manaus. AM, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisa Leônidas e Maria Deane. Manaus. AM, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisa Leônidas e Maria Deane. Manaus. AM, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisa Leônidas e Maria Deane. Manaus. AM, Brasil.
Abstract: The incidence of Shigella spp. was assessed in 877 infants from the public hospital in Rondônia (Western Amazon region, Brazil) where Shigella represents the fourth cause of diarrhea. Twenty-five isolates were identified: 18 were Shigella flexneri, three Shigella sonnei, three Shigella boydii and one Shigella dysenteriae. With the exception of S. dysenteriae, all Shigella spp. isolated from children with diarrhea acquired multiple antibiotic resistances. PCR detection of ipa virulence genes and invasion assays of bloody diarrhea and fever (colitis) were compared among 25 patients testing positive for Shigella. The ipaH and ipaBCD genes were detected in almost all isolates and, unsurprisingly, all Shigella isolates associated with colitis were able to invade HeLa cells. This work alerts for multiple antibiotic resistant Shigella in the region and characterizes presence of ipa virulence genes and invasion phenotypesin dysenteric shigellosis.
Keywords: Shigella spp
Brazil
Multiple antibiotic resistance
Fiarrhea
Ipa genes
Issue Date: 2008
Citation: SILVA, Tatiane et al. Characterization of Shigella spp. by antimicrobial resistance and PCR detection of ipa genes in an infantile population from Porto Velho (Western Amazon region), Brazil. Memória do Instituto Oswaldo Cruz, Rio de Janeiro, v. 103, n. 7, p. 731-733, Nov. 2008.
ISSN: 0074-0276
Copyright: open access
Appears in Collections:AM - ILMD - Artigos de Periódicos

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