Characterization of mycobacteria and mycobacteriophages isolated from compost at the São Paulo Zoo Park Foundation in Brazil and creation of the new mycobacteriophage Cluster U

Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brazil.
Universidade Federal de São Paulo. Campus Diadema. Departamento de Ciências Biológicas. São Paulo, SP, Brazil.
Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brazil.
Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brazil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.
Universidade Federal de São Paulo. Instituto de Química. Departamento de Bioquímica. São Paulo, SP, Brazil.
Universidade Federal de São Paulo. Instituto de Química. Departamento de Bioquímica. São Paulo, SP, Brazil.
Universidade Federal de São Paulo. Escola de Artes, Ciências e Humanidades. São Paulo, SP, Brazil.
Universidade Federal de São Paulo. Instituto de Química. Departamento de Bioquímica. São Paulo, SP, Brazil.
Universidade Federal de São Paulo. Instituto de Química. Departamento de Bioquímica. São Paulo, SP, Brazil / Virginia Bioinformatics Institute. Blacksburg, VA, USA.
University of Pittsburgh. Department of Biological Sciences. Pittsburgh, PA, USA.
University of Pittsburgh. Department of Biological Sciences. Pittsburgh, PA, USA.
University of Pittsburgh. Department of Biological Sciences. Pittsburgh, PA, USA.
University of Pittsburgh. Department of Biological Sciences. Pittsburgh, PA, USA.
Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brazil.

Abstract

Background: A large collection of sequenced mycobacteriophages capable of infecting a single host strain of Mycobacterium smegmatis shows considerable genomic diversity with dozens of distinctive types (clusters) and extensive variation within those sharing evident nucleotide sequence similarity. Here we profiled the mycobacterial components of a large composting system at the São Paulo zoo. Results: We isolated and sequenced eight mycobacteriophages using Mycobacterium smegmatis mc2 155 as a host. None of these eight phages infected any of mycobacterial strains isolated from the same materials. The phage isolates span considerable genomic diversity, including two phages (Barriga, Nhonho) related to Subcluster A1 phages, two Cluster B phages (Pops, Subcluster B1; Godines, Subcluster B2), three Subcluster F1 phages (Florinda, Girafales, and Quico), and Madruga, a relative of phage Patience with which it constitutes the new Cluster U. Interestingly, the two Subcluster A1 phages and the three Subcluster F1 phages have genomic relationships indicating relatively recent evolution within a geographically isolated niche in the composting system. Conclusions: We predict that composting systems such as those used to obtain these mycobacteriophages will be a rich source for the isolation of additional phages that will expand our view of bacteriophage diversity and evolution.

Citation

LIMA-JUNIOR, J. D. et al. Characterization of mycobacteria and mycobacteriophages isolated from compost at the São Paulo Zoo Park Foundation in Brazil and creation of the new mycobacteriophage Cluster U. BMC Microbiology, v. 16, n. 1, p. 1-15, Dec. 2016.

DOI

10.1186/s12866-016-0734-3

ISSN

1471-2180

Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/19340

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