Author | Neves, Neuza Maria Alcantara | |
Author | Veiga, Rafael Valente | |
Author | Ponte, João Carlos Marques | |
Author | Cunha, Sérgio Souza da | |
Author | Simões, Silvia de Magalhães | |
Author | Cruz Filho, Álvaro Augusto Souza da | |
Author | Yazdanbakhsh, Maria | |
Author | Matos, Sheila Maria Alvim de | |
Author | Silva, Thiago Magalhães da | |
Author | Figueiredo, Camila A | |
Author | Pontes-de-Carvalho, Lain Carlos | |
Author | Rodrigues, Laura C | |
Author | Fiaccone, Rosemeire Leovigildo | |
Author | Cooper, Philip J | |
Author | Barreto, Maurício Lima | |
Access date | 2017-06-12T14:01:00Z | |
Available date | 2017-06-12T14:01:00Z | |
Document date | 2017 | |
Citation | NEVES, N. M. A. et al. Dissociation between skin test reactivity and anti-aeroallergen IgE: Determinants among urban Brazilian children. Plos One, 2017 | pt_BR |
ISSN | 1932-6203 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/19364 | |
Sponsorship | HCPC Latin America Excellence Centre Programme (072405/Z/03/Z) and grant 088862/Z/09/Z; and INCT/MCT/CNPq Programme (Contract no. 5737862008-9). We thank CAPES, CNPq and FAPESB | pt_BR |
Language | eng | pt_BR |
Publisher | Public Library of Science | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Testes cutâneos | pt_BR |
Subject in Portuguese | Alergia | pt_BR |
Subject in Portuguese | Alergenos | pt_BR |
Subject in Portuguese | Meio ambiente | pt_BR |
Subject in Portuguese | Higiene | pt_BR |
Subject in Portuguese | Humanos | pt_BR |
Subject in Portuguese | Crianças | pt_BR |
Subject in Portuguese | Brazil | pt_BR |
Title | Dissociation between skin test reactivity and anti-aeroallergen IgE: Determinants among urban Brazilian children | pt_BR |
Type | Article | pt_BR |
DOI | 10.1371/journal.pone.0174089 | |
Abstract | The dissociation between specific IgE and skin prick test reactivity to aeroallergens, a common finding in populations living in low and middle-income countries, has important implications for the diagnosis and treatment of allergic diseases. Few studies have investigated the determinants of this dissociation. In the present study, we explored potential factors explaining this dissociation in children living in an urban area of Northeast Brazil, focusing in particular on factors associated with poor hygiene. Methods
Of 1445 children from low income communities, investigated for risk factors of allergies, we
studied 481 with specific IgE antibodies to any of Blomia tropicalis, Dermatophagoides pteronyssinus,
Periplaneta americana and Blatella germanica allergens. Data on demographic,
environmental and social exposures were collected by questionnaire; serum IgG and stool
examinations were done to detect current or past infections with viral, bacterial, protozoan
and intestinal helminth pathogens. We measured atopy by skin prick testing (SPT) and specific
IgE (sIgE) to aerollergens in serum (by ImmunoCAP). SIgE reactivity to B. tropicalis
extract depleted of carbohydrates was measured by an in-house ELISA. Total IgE was measured
by in house capture ELISA. SNPs were typed using Illumina Omni 2.5. Results
Negative skin prick tests in the presence of specific IgE antibodies were frequent. Factors
independently associated with a reduced frequency of positive skin prick tests were large
number of siblings, the presence of IgG to herpes simplex virus, Ascaris lumbricoides and
Trichuris trichiura infections, living in neighborhoods with infrequent garbage collection,
presence of rodents and cats in the household and sIgE reactivity to glycosylated B. tropicalis
allergens. Also, SNP on IGHE (rs61737468) was negatively associated with SPT
reactivity.
Conclusions
A variety of factors were found to be associated with decreased frequency of SPT such as
unhygienic living conditions, infections, total IgE, IgE response to glycosylated allergens
and genetic polymorphisms, indicating that multiple mechanisms may be involved. Our
data, showing that exposures to an unhygienic environment and childhood infections modulate
immediate allergen skin test reactivity, provide support for the "hygiene hypothesis". | pt_BR |
Affilliation | Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil / Universidade Federal de Juiz de Fora. Juiz de Fora, MG, Brasil | pt_BR |
Affilliation | Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade Federal de Pernambuco. Departamento Medicina Social. Recife, PE, Brasil | pt_BR |
Affilliation | Universidade Federal de Sergipe. Departamento de Pediatria. Aracaju, SE, Brasil | pt_BR |
Affilliation | Universidade Federal da Bahia. ProAR- Núcleo de Excelência em Asma. Salvador, BA, Brasil | pt_BR |
Affilliation | Leiden University Medical Center da Bahia. Department of Parasitology. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade Federal da Bahia. Instituto de Saúde Coletiva. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade Federal da Bahia. Instituto de Saúde Coletiva. Salvador, BA, Brasil / Universidade Estadual do Sudoeste da Bahia. Departamento de Ciências Biológicas. Vitória da Conquista, BA, Brasil | pt_BR |
Affilliation | Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | St George's University of London. Institute of Infection and Immunity. London, United Kingdom, | pt_BR |
Affilliation | Universidade Federal da Bahia. Instituto de Matemática. Departamento de Estatística. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidad Internacional del Ecuador. Faculty of Facultad de Ciencias Medicas, de la Salud y la Vida. Quito, Ecuador / St George's University of London. Institute of Infection and Immunity. London, United Kingdom | pt_BR |
Affilliation | Universidade Federal da Bahia. Instituto de Saúde Coletiva. Salvador, BA, Brasil | pt_BR |
Subject | Skin test | pt_BR |
Subject | Allergy | pt_BR |
Subject | Allergens | pt_BR |
Subject | Environmental | pt_BR |
Subject | Hygiene | pt_BR |
Subject | Humans | pt_BR |
Subject | Children | pt_BR |
Subject | Brazil | pt_BR |
DeCS | Testes cutâneos | pt_BR |
DeCS | Alergia | pt_BR |
DeCS | Alergenos | pt_BR |
DeCS | Meio ambiente | pt_BR |
DeCS | Higiene | pt_BR |
DeCS | Humanos | pt_BR |
DeCS | Crianças | pt_BR |
DeCS | Brazi | pt_BR |