Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/19364
Title: Dissociation between skin test reactivity and anti-aeroallergen IgE: Determinants among urban Brazilian children
Authors: Neves, Neuza Maria Alcantara
Veiga, Rafael Valente
Ponte, João Carlos Marques
Cunha, Sérgio Souza da
Simões, Silvia de Magalhães
Cruz Filho, Álvaro Augusto Souza da
Yazdanbakhsh, Maria
Matos, Sheila Maria Alvim de
Silva, Thiago Magalhães da
Figueiredo, Camila A
Pontes-de-Carvalho, Lain Carlos
Rodrigues, Laura C
Fiaccone, Rosemeire Leovigildo
Cooper, Philip J
Barreto, Maurício Lima
Affilliation: Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil
Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil / Universidade Federal de Juiz de Fora. Juiz de Fora, MG, Brasil
Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil
Universidade Federal de Pernambuco. Departamento Medicina Social. Recife, PE, Brasil
Universidade Federal de Sergipe. Departamento de Pediatria. Aracaju, SE, Brasil
Universidade Federal da Bahia. ProAR- Núcleo de Excelência em Asma. Salvador, BA, Brasil
Leiden University Medical Center da Bahia. Department of Parasitology. Salvador, BA, Brasil
Universidade Federal da Bahia. Instituto de Saúde Coletiva. Salvador, BA, Brasil
Universidade Federal da Bahia. Instituto de Saúde Coletiva. Salvador, BA, Brasil / Universidade Estadual do Sudoeste da Bahia. Departamento de Ciências Biológicas. Vitória da Conquista, BA, Brasil
Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
St George's University of London. Institute of Infection and Immunity. London, United Kingdom,
Universidade Federal da Bahia. Instituto de Matemática. Departamento de Estatística. Salvador, BA, Brasil
Universidad Internacional del Ecuador. Faculty of Facultad de Ciencias Medicas, de la Salud y la Vida. Quito, Ecuador / St George's University of London. Institute of Infection and Immunity. London, United Kingdom
Universidade Federal da Bahia. Instituto de Saúde Coletiva. Salvador, BA, Brasil
Abstract: The dissociation between specific IgE and skin prick test reactivity to aeroallergens, a common finding in populations living in low and middle-income countries, has important implications for the diagnosis and treatment of allergic diseases. Few studies have investigated the determinants of this dissociation. In the present study, we explored potential factors explaining this dissociation in children living in an urban area of Northeast Brazil, focusing in particular on factors associated with poor hygiene. Methods Of 1445 children from low income communities, investigated for risk factors of allergies, we studied 481 with specific IgE antibodies to any of Blomia tropicalis, Dermatophagoides pteronyssinus, Periplaneta americana and Blatella germanica allergens. Data on demographic, environmental and social exposures were collected by questionnaire; serum IgG and stool examinations were done to detect current or past infections with viral, bacterial, protozoan and intestinal helminth pathogens. We measured atopy by skin prick testing (SPT) and specific IgE (sIgE) to aerollergens in serum (by ImmunoCAP). SIgE reactivity to B. tropicalis extract depleted of carbohydrates was measured by an in-house ELISA. Total IgE was measured by in house capture ELISA. SNPs were typed using Illumina Omni 2.5. Results Negative skin prick tests in the presence of specific IgE antibodies were frequent. Factors independently associated with a reduced frequency of positive skin prick tests were large number of siblings, the presence of IgG to herpes simplex virus, Ascaris lumbricoides and Trichuris trichiura infections, living in neighborhoods with infrequent garbage collection, presence of rodents and cats in the household and sIgE reactivity to glycosylated B. tropicalis allergens. Also, SNP on IGHE (rs61737468) was negatively associated with SPT reactivity. Conclusions A variety of factors were found to be associated with decreased frequency of SPT such as unhygienic living conditions, infections, total IgE, IgE response to glycosylated allergens and genetic polymorphisms, indicating that multiple mechanisms may be involved. Our data, showing that exposures to an unhygienic environment and childhood infections modulate immediate allergen skin test reactivity, provide support for the "hygiene hypothesis".
Keywords: Skin test
Allergy
Allergens
Environmental
Hygiene
Humans
Children
Brazil
keywords: Testes cutâneos
Alergia
Alergenos
Meio ambiente
Higiene
Humanos
Crianças
Brazil
DeCS: Testes cutâneos
Alergia
Alergenos
Meio ambiente
Higiene
Humanos
Crianças
Brazi
Issue Date: 2017
Publisher: Public Library of Science
Citation: NEVES, N. M. A. et al. Dissociation between skin test reactivity and anti-aeroallergen IgE: Determinants among urban Brazilian children. Plos One, 2017
DOI: 10.1371/journal.pone.0174089
ISSN: 1932-6203
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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