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THE MITOCHONDRIAL GENOME OF THE PHYTOPATHOGENIC BASIDIOMYCETE MONILIOPHTHORA PERNICIOSA IS 109 KB IN SIZE AND CONTAINS A STABLE INTEGRATED PLASMID
Formighieri, Eduardo Fernandes et al. | Date Issued: 2008
Author
Formighieri, Eduardo Fernandes
Tiburcio, Ricardo A
Armas, Eduardo Dutra de
Medrano, Francisco Javier
Shimo, Hugo
Carels, Nicolas
Góes Neto, Aristóteles
Cotomacci, Carolina
Carazzolle, Marcelo Falsarella
Pinto, Naiara Sardinha
Thomazella, Daniela Paula de Toledo
Rincones, Johana
Digiampietri, Luciano
Carraro, Dirce Maria
Espin, Ana Maria Lima de Azeredo
Reis, Sérgio Furtado dos
Deckmann, Ana Carolina
Gramacho, Karina
Gonçalves, Marilda de Souza
Moura Neto, José Pereira de
Barbosa, Luciana Veiga
Meinhardt, Lyndel W
Cascardo, Júlio Cezar de Mattos
Pereira, Gonçalo Amarante Guimarães
Affilliation
Múltipla – ver em Notas
Abstract
We present here the sequence of the mitochondrial genome of the basidiomycete phytopathogenic hemibiotrophic fungus Moniliophthora perniciosa, causal agent of the Witches' Broom Disease in Theobroma cacao. The DNA is a circular molecule of 109,103 base pairs, with 31.9% GC, and is the largest sequenced so far. This size is due essentially to the presence of numerous non-conserved hypothetical ORFs. It contains the 14 genes coding for proteins involved in the oxidative phosphorylation, the two rRNA genes, one ORF coding for a ribosomal protein (rps3), and a set of 26 tRNA genes that recognize codons for all amino acids. Seven homing endonucleases are located inside introns. Except atp8, all conserved known genes are in the same orientation. Phylogenetic analysis based on the cox genes agrees with the commonly accepted fungal taxonomy. An uncommon feature of this mitochondrial genome is the presence of a region that contains a set of four, relatively small, nested, inverted repeats enclosing two genes coding for polymerases with an invertron-type structure and three conserved hypothetical genes interpreted as the stable integration of a mitochondrial linear plasmid. The integration of this plasmid seems to be a recent evolutionary event that could have implications in fungal biology. This sequence is available under GenBank accession number AY376688.
Keywords in Portuguese
Basidiomycota
Uso de códon
Crinipellis perniciosa
Genômica
Marasmiaceae
Doença das vassouras das bruxas
 
Keywords
Basidiomycota
Codon usage
Crinipellis perniciosa
Genomics
Marasmiaceae
Witches’ broom disease
 
Publisher
Elsevier
Citation
FORMIGHIERI, E. F. et al. The mitochondrial genome of the phytopathogenic basidiomycete Moniliophthora perniciosa is 109 kb in size and contains a stable integrated plasmid. Mycological Research, v. 112, p. 1136–1152, 2008.
DOI
10.1016/j.mycres.2008.04.014
ISSN
0953-7562
Notes
Gonçalves, Marilda de Souza Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”. aLaboratório de Genômica e Expressão – Departamento de Genética e Evolução, Instituto de Biologia, Universidade Estadual de Campinas, 13083-970, Campinas – SP, Brazil bLaboratório de Ecotoxicologia, Centro de Energia Nuclear na Agricultura, Universidade de São Paulo, 13400-970, Piracicaba – SP, Brazil cLaboratório de Bioinformatica da Universidade Estadual de Santa Cruz, 45650-000, Ilhéus – BA, Brazil dLaboratório de Pesquisa em Microbiologia (LAPEM), Departamento de Ciências Biológicas, Universidade Estadual de Feira de Santana (UEFS), 44031-460, Feira de Santana – BA, Brazil eInstituto de Computação, Universidade Estadual de Campinas, 13084-971, Campinas – SP, Brazil fLudwig Institute For Cancer Research, 01509-010, São Paulo – SP, Brazil gDepartamento de Genética e Evolução e Laboratório de Genética Animal, Centro de Biologia Molecular e Engenharia Genética – CBMEG, Universidade Estadual de Campinas, 13035-875, Campinas – SP, Brazil hDepartamento de Parasitologia, Instituto de Biologia, Universidade Estadual de Campinas, 13083-970, Campinas – SP, Brazil iCEPLAC/CEPEC/SEFIT, 45600-970, Itabuna – BA, Brazil jLaboratoório de Biologia Molecular – Faculdade de Farmácia, Universidade Federal da Bahia, 40170-290, Salvador – BA, Brazil kLaboratório de Biologia Molecular – Departamento de Biologia Geral, Instituto de Biologia, Universidade Federal da Bahia, 40170-290, Salvador – BA, Brazil lSustainable Perennial Crops Laboratory, USDA-ARS, 10300 Baltimore, Av., BARC-W, Beltsville, MD 20740, USA mDepartamento de Ciências Biológicas, Universidade Estadual de Santa Cruz, 45650-000, Ilhéus – BA, Brazil