Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/19432
Title: Performance of Molecular Methods for Hepatitis C Virus Diagnosis: Usefulness among Chronic Cases and during the Course of Infection
Authors: Martins, Patricia Pais
Lampe, Elisabeth
Lewis-Ximenez, Lia Laura
Souza, Paulo Sergio Fonseca de
Fernandes, Carlos Augusto
Villar, Livia Melo
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ. Brasil.
Laboratório Central de Saúde Pública Noel Nutels. Divisão de Hepatites. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ. Brasil.
Abstract: Background: Molecular methods are essential to define hepatitis C virus (HCV) infection. This study was conducted to evaluate the performance of molecular qualitative and quantitative methods for HCV RNA among chronic patients and individuals during the course of HCV infection. Methods: Single serum samples were obtained from 82 HCV infected individuals where six of them donated serial serum samples (n = 52) during the course of HCV infection. Qualitative (in-house RT-nested PCR and COBAS® AMPLICOR HCV Test v2.0 and TMA) and quantitative (COBAS® AMPLICOR HCV Monitor Test v2.0 and bDNA) techniques were employed. Results: TMA presented the highest rate (87.8%) of HCV detection among qualitative tests and it was the most sensitive for HCV RNA detection during the early and late phases of HCV infection. HCV RNA was quantified among 56 samples and significant correlation was observed between the two assays (r 0.92; p < 0.0001). Conclusions: It is concluded that both quantitative methods can be used among chronic and acute HCV cases, but TMA was the most efficient for HCV qualitative detection among chronic cases and in the early and late phases of HCV infection.
Keywords: Hepatitis C
molecular diagnosis
performance
keywords: Hepatite C
Diagnóstico molecular
Performance
Issue Date: 2013
Publisher: Clinical Laboratory Publications
Citation: MARTINS, Patricia Pais; et al. Performance of Molecular Methods for Hepatitis C Virus Diagnosis: Usefulness among Chronic Cases and during the Course of Infection. Clin. Lab. v..59, p.1031-1039, 2013.
DOI: 10.7754/Clin.Lab.2013.120903
ISSN: 1433-6510
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

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