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AutorChagas Júnior, Adenizar Delgado das
AutorMcBride, Alan John Alexander
AutorAthanazio, Daniel Abensur
AutorFigueira, Cláudio Pereira
AutorMedeiros, Marco Alberto
AutorReis, Mitermayer Galvão dos
AutorKo, Albert Icksang
AutorMcBride, Flávia Weykamp da Cruz
Fecha de acceso2011-04-25T19:02:54Z
Fecha de disponibilización2011-04-25T19:02:54Z
Fecha de publicación2009
ReferenciaCHAGAS-JUNIOR, A. D. et al. An Imprint method for detecting leptospires in the hamster model of vaccine-mediated immunity for leptospirosis. Journal of Medical Microbiology, v. 58, n.12, p.1632–1637, 2009.pt_BR
ISSN0022-2615
URIhttps://www.arca.fiocruz.br/handle/icict/1960
Idiomaengpt_BR
Derechos de autoropen access
TítuloAn Imprint method for detecting leptospires in the hamster model of vaccine-mediated immunity for leptospirosispt_BR
Tipo del documentoArticlept_BR
DOI10.1099/jmm.0.014050-0
Resumen en InglésIn determining the efficacy of new vaccine candidates for leptospirosis, the primary end point is death and an important secondary end point is sterilizing immunity. However, evaluation of this end point is often hampered by the time-consuming demands and complexity of methods such as culture isolation (CI). In this study, we evaluated the use of an imprint (or touch preparation) method (IM) in detecting the presence of leptospires in tissues of hamsters infected with Leptospira interrogans serovar Copenhageni. In a dissemination study, compared to CI, the IM led to equal or improved detection of leptospires in kidney, liver, lung and blood samples collected post-infection and overall concordance was good (kappa=0.61). Furthermore, in an evaluation of hamsters immunized with a recombinant leptospiral protein-based vaccine candidate and subsequently challenged, the agreement between the CI and IM was very good (kappa=0.84). These findings indicate that the IM is a rapid method for the direct observation of Leptospira spp. that can be readily applied to evaluating infection in experimental animals and determining sterilizing immunity when screening potential vaccine candidates.pt_BR
AfiliaciónFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasilpt_BR
AfiliaciónFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasilpt_BR
AfiliaciónFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasilpt_BR
AfiliaciónFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasilpt_BR
AfiliaciónFundação Oswaldo Cruz. Bio-Manguinhos, Rio de Janeiro, RJ, Brasilpt_BR
AfiliaciónFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasilpt_BR
AfiliaciónFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasilpt_BR
AfiliaciónFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasilpt_BR
DeCSLeptospira interroganspt_BR
DeCSVacinas Bacterianaspt_BR
DeCSLeptospirosept_BR
DeCSAnimaispt_BR
DeCSSensibilidade e Especificidadept_BR
DeCSCricetinaept_BR


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