Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/20080
Title: Phenotypic evaluation and in silico ADMET properties of novel arylimidamides in acute mouse models of Trypanosoma cruzi infection
Authors: Silva, Cristiane França da
Batista, Denise da Gama Jaén
Araújo, Julianna Sicilano de
Cunha Junior, Edézio Ferreira
Stephens, Chad E.
Banerjee, Moloy
Farahat, Abdelbasset A.
Akay, Senol
Fisher, Mary K,
Boykin, David W.
Soeiro, Maria de Nazaré Correia
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica de Tripanossomatídeos. Rio de Janeiro, RJ. Brasil.
Augusta University. Department of Chemistry and Physics. Augusta, USA.
Georgia State University. Department of Chemistry. Atlanta, GA, USA.
Georgia State University. Department of Chemistry. Atlanta, GA, USA / Mansoura University. Faculty of Pharmacy. Department of Pharmaceutical Organic Chemistry. Mansoura, Egypt.
Georgia State University. Department of Chemistry. Atlanta, GA, USA.
Augusta University. Department of Chemistry and Physics. Augusta, USA.
Georgia State University. Department of Chemistry. Atlanta, GA, USA.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ. Brasil.
Abstract: Abstract: Arylimidamides (AIAs), previously termed as reversed amidines, present a broad spectrum of activity against intracellular microorganisms. In the present study, three novel AIAs were evaluated in a mouse model of Trypanosoma cruzi infection, which is the causative agent of Chagas disease. The bis-AIAs DB1957, DB1959 and DB1890 were chosen based on a previous screening of their scaffolds that revealed a very promising trypanocidal effect at nanomolar range against both the bloodstream trypomastigotes (BTs) and the intracellular forms of the parasite. This study focused on both mesylate salts DB1957 and DB1959 besides the hydrochloride salt DB1890. Our current data validate the high activity of these bis-AIA scaffolds that exhibited EC50 (drug concentration that reduces 50% of the number of the treated parasites) values ranging from 14 to 78 nM and 190 to 1,090 nM against bloodstream and intracellular forms, respectively, also presenting reasonable selectivity indexes and no mutagenicity profile predicted by in silico absorption, distribution, metabolism, excretion, and toxicity (ADMET). Acute toxicity studies using murine models revealed that these AIAs presented only mild toxic effects such as reversible abdominal contractions and ruffled fur. Efficacy assays performed with Swiss mice infected with the Y strain revealed that the administration of DB1957 for 5 consecutive days, with the first dose given at parasitemia onset, reduced the number of BTs at the peak, ranging between 21 and 31% of decrease. DB1957 was able to provide 100% of animal survival, while untreated animals showed 70% of mortality rates. DB1959 and DB1890B did not reduce circulating parasitism but yielded .80% of survival rates.
Keywords: Chagas disease
arylimidamides
experimental chemotherapy
in vivo assays
keywords: Doença de Chagas
Quimioterapia experimental
Ensaios in vivo
Arilimidamidas
Issue Date: 2017
Publisher: Dove Medical Press
Citation: SILVA, Cristiana França da; et al Phenotypic evaluation and in silico ADMET properties of novel arylimidamides in acute mouse models of Trypanosoma cruzi infection. Drug Design, Development and Therapy, v.11, p.1095-1105, 2017.
ISSN: 1177-8881
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

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