| Autor | Kalb, Ligia Cristina | |
| Autor | Frederico, Yohana Camila Antunes | |
| Autor | Boehm, Corrdula | |
| Autor | Moreira, Claudia Maria do Nascimento | |
| Autor | Soares, Maurilio José | |
| Autor | Field, Mark C. | |
| Data de acesso | 2017-07-27T12:05:39Z | |
| Data de disponibilização | 2017-07-27T12:05:39Z | |
| Data do publicação | 2016 | |
| Citação | KALB, Ligia Cristina et al. Conservation and divergence within the clathrin interactome of Trypanosoma cruzi. Scientific Reports v. 6, n. 31212, p. 1-12. 9 Aug. 2016. | pt_BR |
| ISSN | 2045-2322 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/20421 | |
| Idioma | eng | pt_BR |
| Editor | Nature Publishing Group | pt_BR |
| Direito Autoral | open access | pt_BR |
| Palavras-chave | Trypanosoma cruzi | pt_BR |
| Título | Conservation and divergence within the Clathrin interactome of Trypanosoma cruzi | pt_BR |
| Tipo do documento | Article | pt_BR |
| DOI | 10.1038/srep31212 | pt_BR |
| Resumo em Inglês | Trypanosomatids are parasitic protozoa with a significant burden on human health. African and American trypanosomes are causative agents of Nagana and Chagas disease respectively, and speciated about 300 million years ago. These parasites have highly distinct life cycles, pathologies, transmission strategies and surface proteomes, being dominated by the variant surface glycoprotein (African) or mucins (American) respectively. In African trypanosomes clathrin-mediated trafficking is responsible for endocytosis and post-Golgi transport, with several mechanistic aspects distinct from higher organisms. Using clathrin light chain (TcCLC) and EpsinR (TcEpsinR) as affinity handles, we identified candidate clathrin-associated proteins (CAPs) in Trypanosoma cruzi; the cohort includes orthologs of many proteins known to mediate vesicle trafficking, but significantly not the AP-2 adaptor complex. Several trypanosome-specific proteins common with African trypanosomes, were also identified. Fluorescence microscopy revealed localisations for TcEpsinR, TcCLC and TcCHC at the posterior region of trypomastigote cells, coincident with the flagellar pocket and Golgi apparatus. These data provide the first systematic analysis of clathrin-mediated trafficking in T. cruzi, allowing comparison between protein cohorts and other trypanosomes and also suggest that clathrin trafficking in at least some life stages of T. cruzi may be AP-2-independent. | pt_BR |
| Afiliação | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Celular, Curitiba, PR, Brasil / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Molecular de Tripanosomas, Curitiba, PR, Brasil. | pt_BR |
| Afiliação | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Celular, Curitiba, PR, Brasil. | pt_BR |
| Afiliação | School of Life Sciences. University of Dundee. Dundee, UK. | pt_BR |
| Afiliação | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Molecular de Tripanosomas, Curitiba, PR, Brasil / School of Life Sciences. University of Dundee. Dundee, UK. | pt_BR |
| Afiliação | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Celular, Curitiba, PR, Brasil. | pt_BR |
| Afiliação | School of Life Sciences. University of Dundee. Dundee, UK. | pt_BR |
| Palavras-chave em inglês | Endocytosis | pt_BR |
| Palavras-chave em inglês | Clathrin | pt_BR |
| Palavras-chave em inglês | Adaptor Protein Complex 2 | pt_BR |
| Palavras-chave em espanhol | Endocitosis | pt_BR |
| Palavras-chave em espanhol | Clatrina | pt_BR |
| Palavras-chave em espanhol | Complejo 2 de Proteína Adaptadora | pt_BR |
| DeCS | Trypanosoma cruzi | pt_BR |
| DeCS | Endocitose | pt_BR |
| DeCS | Clatrina | pt_BR |
| DeCS | Complexo 2 de Proteínas Adaptadoras | pt_BR |
