Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/20421
Title: Conservation and divergence within the Clathrin interactome of Trypanosoma cruzi
Authors: Kalb, Ligia Cristina
Frederico, Yohana Camila Antunes
Boehm, Corrdula
Moreira, Claudia Maria do Nascimento
Soares, Maurilio Jose
Field, Mark C.
Affilliation: Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Celular, Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Molecular de Tripanosomas, Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Celular, Curitiba, PR, Brasil.
School of Life Sciences. University of Dundee. Dundee, UK.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Molecular de Tripanosomas, Curitiba, PR, Brasil. / School of Life Sciences. University of Dundee. Dundee, UK.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Celular, Curitiba, PR, Brasil.
School of Life Sciences. University of Dundee. Dundee, UK.
Abstract: Trypanosomatids are parasitic protozoa with a significant burden on human health. African and American trypanosomes are causative agents of Nagana and Chagas disease respectively, and speciated about 300 million years ago. These parasites have highly distinct life cycles, pathologies, transmission strategies and surface proteomes, being dominated by the variant surface glycoprotein (African) or mucins (American) respectively. In African trypanosomes clathrin-mediated trafficking is responsible for endocytosis and post-Golgi transport, with several mechanistic aspects distinct from higher organisms. Using clathrin light chain (TcCLC) and EpsinR (TcEpsinR) as affinity handles, we identified candidate clathrin-associated proteins (CAPs) in Trypanosoma cruzi; the cohort includes orthologs of many proteins known to mediate vesicle trafficking, but significantly not the AP-2 adaptor complex. Several trypanosome-specific proteins common with African trypanosomes, were also identified. Fluorescence microscopy revealed localisations for TcEpsinR, TcCLC and TcCHC at the posterior region of trypomastigote cells, coincident with the flagellar pocket and Golgi apparatus. These data provide the first systematic analysis of clathrin-mediated trafficking in T. cruzi, allowing comparison between protein cohorts and other trypanosomes and also suggest that clathrin trafficking in at least some life stages of T. cruzi may be AP-2-independent.
Keywords: Endocytosis
Clathrin
Adaptor Protein Complex 2
Keywords in spanish: Endocitosis
Clatrina
Complejo 2 de Proteína Adaptadora
keywords: Trypanosoma cruzi
DeCS: Trypanosoma cruzi
Endocitose
Clatrina
Complexo 2 de Proteínas Adaptadoras
Issue Date: 2016
Publisher: Nature Publishing Group
Citation: KALB, Ligia Cristina et al. Conservation and divergence within the clathrin interactome of Trypanosoma cruzi. Scientific Reports v. 6, n. 31212, p. 1-12. 9 Aug. 2016.
DOI: doi: 10.1038/srep31212
ISSN: 2045-2322
Copyright: open access
Appears in Collections:PR - ICC - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
CONSERVATION AND DIVERGENCE WITHIN THE CLATHRIN INTERACTOME OF TRYPANOSOMA CRUZI.pdf1.03 MBAdobe PDFView/Open


FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.