| Author | Gravina, Humberto Doriguêtto | |
| Author | Goes, Alfredo Miranda | |
| Author | Murta, Silvane Maria Fonseca | |
| Author | Ropert, Catherine | |
| Access date | 2017-08-10T18:09:27Z | |
| Available date | 2017-08-10T18:09:27Z | |
| Document date | 2016 | |
| Citation | GRAVINA, Humberto Doriguêtto et al. MyD88 Adapter-like (Mal)/TIRAP Is Required for Cytokine Production by Splenic Ly6C(lo)TLR2(hi) but Not by Ly6C(hi)TLR2(hi) Monocytes during Trypanosoma cruzi Infection. J Biol Chem., v. 291, n. 45, p. 23832-23841. | pt_BR |
| ISSN | 0021-9258 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/20575 | |
| Language | eng | pt_BR |
| Publisher | American Society for Biochemistry and Molecular Biology | pt_BR |
| Rights | restricted access | pt_BR |
| Subject in Portuguese | cytokine | pt_BR |
| Title | MyD88 Adapter-like (Mal)/TIRAP Is Required for Cytokine Production by Splenic Ly6C(lo)TLR2(hi) but Not by Ly6C(hi)TLR2(hi) Monocytes during Trypanosoma cruzi Infection. | pt_BR |
| Type | Article | |
| Abstract | This study continues to explore the plasticity of Toll-like receptor 2 (TLR2) previously described in immune response during Trypanosoma cruzi infection. Here, we have shown that Ly6ChiTLR2hi monocytes were involved in TNF-α and IL-12 production, whereas Ly6CloTLR2hi monocytes were mainly committed to IL-10 and TNF-α production during T. cruzi infection independently of TLR agonist used (i.e. TLR2 or TLR9 agonists). Another difference between the monocyte populations is that the adapter Mal (encoded by TIRAP) has appeared crucial for the cytokine production by Ly6Clo but not by Ly6Chi monocytes. The protein Mal was necessary to induce cytokine synthesis by Ly6Clo monocytes after triggering TLR2 or TLR9. Finally, our data have suggested that TLR2, TLR9, and Mal/TIRAP controlled differentially the emergence of the different TLR2hi monocyte populations in the spleen. In summary, this study highlights the central role of the TLR2/Mal tandem in the distinct activity among the monocyte subsets during T. cruzi infection. Such findings provide a basis for understanding the challenge posed by the use of TLR2 agonist in immunotherapy. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil | pt_BR |
| Subject | cytokine | pt_BR |
| Subject | flow cytometry | pt_BR |
| Subject | infection | pt_BR |
| Subject | inflammation | pt_BR |
| Subject | innate immunity | pt_BR |
| Subject | MAL/TIRAP | pt_BR |
| Subject | monocyte | pt_BR |
| Subject | Toll-like receptor (TLR) | pt_BR |
| Embargo date | 2028-01-01 | |
