Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/20575
Title: MyD88 Adapter-like (Mal)/TIRAP Is Required for Cytokine Production by Splenic Ly6C(lo)TLR2(hi) but Not by Ly6C(hi)TLR2(hi) Monocytes during Trypanosoma cruzi Infection.
Authors: Gravina, Humberto Doriguêtto
Goes, Alfredo Miranda
Murta, Silvane Maria Fonseca
Ropert, Catherine
Affilliation: Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil
Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil.
Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil
Abstract: This study continues to explore the plasticity of Toll-like receptor 2 (TLR2) previously described in immune response during Trypanosoma cruzi infection. Here, we have shown that Ly6ChiTLR2hi monocytes were involved in TNF-α and IL-12 production, whereas Ly6CloTLR2hi monocytes were mainly committed to IL-10 and TNF-α production during T. cruzi infection independently of TLR agonist used (i.e. TLR2 or TLR9 agonists). Another difference between the monocyte populations is that the adapter Mal (encoded by TIRAP) has appeared crucial for the cytokine production by Ly6Clo but not by Ly6Chi monocytes. The protein Mal was necessary to induce cytokine synthesis by Ly6Clo monocytes after triggering TLR2 or TLR9. Finally, our data have suggested that TLR2, TLR9, and Mal/TIRAP controlled differentially the emergence of the different TLR2hi monocyte populations in the spleen. In summary, this study highlights the central role of the TLR2/Mal tandem in the distinct activity among the monocyte subsets during T. cruzi infection. Such findings provide a basis for understanding the challenge posed by the use of TLR2 agonist in immunotherapy.
Keywords: cytokine
flow cytometry
infection
inflammation
innate immunity
MAL/TIRAP
monocyte
Toll-like receptor (TLR)
keywords: cytokine
Issue Date: 2016
Publisher: American Society for Biochemistry and Molecular Biology
Citation: GRAVINA, Humberto Doriguêtto et al. MyD88 Adapter-like (Mal)/TIRAP Is Required for Cytokine Production by Splenic Ly6C(lo)TLR2(hi) but Not by Ly6C(hi)TLR2(hi) Monocytes during Trypanosoma cruzi Infection. J Biol Chem., v. 291, n. 45, p. 23832-23841.
ISSN: 0021-9258
Copyright: restricted access
Appears in Collections:MG - IRR - Artigos de Periódicos

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