Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/20639
Title: Leishmanicidal effect of antiparasitic photodynamic therapy-ApPDT on infected macrophages
Authors: Oliveira, Susana de
Trahamane, Evaristo João da Ordem
Monteiro, Juliana
Santos, Gustavo Pires
Crugeira, Pedro
Sampaio, Fernando
Oliveira, Camila Indiani de
Barral Netto, Manoel
Pinheiro, Antônio
Affilliation: Federal University of Bahia. Center of Biophotonics. Salvador, BA, Brazil
Federal University of Bahia. Center of Biophotonics. Salvador, BA, Brazil
Federal University of Bahia. Center of Biophotonics. Salvador, BA, Brazil / Estate University of Feira de Santana. Department of Biology. Feira de Santana, Brazil
Federal University of Bahia. Center of Biophotonics. Salvador, BA, Brazil
Federal University of Bahia. Center of Biophotonics. Salvador, BA, Brazil
Federal University of Bahia. Center of Biophotonics. Salvador, BA, Brazil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Federal University of Bahia. Center of Biophotonics. Salvador, BA, Brazil / National Institute of Basic Optics and Applied to Life Sciences. São Carlos, SP, Brazil
Abstract: The aim of this study is to evaluate the effects of ApPDT (antiparasitic photodynamic therapy) on the interaction of Leishmania braziliensis with J774 macrophages, used as a photosensitizer, methylene blue associated with red laser. The tests are in triplicate and the samples divided into four groups: control, photosensitizer, laser, and ApPDT. The photosensitizer used was the methylene blue at concentration of 12.5 μg/mL. The parameters of the laser were λ = 660 nm, 40 mW, and 8.4 J/cm(2). Samples are analyzed by optical microscopy through the identification and counting of infected and uninfected macrophages, parasite load, infectivity, and infection index. Statistical analysis used ANOVA test with Tukey post-test, being considered statistically significant p < 0.05. The analysis of the interaction tests shows that the infection rate in the ApPDT group in relation to the control group presents a statistically significant reduction (p < 0.0001) of 71% at both 24 and 48 h (p < 0.0001) of 62%. ApPDT reduces the number of macrophages infected by Leishmania braziliensis, as well as the number of intracellular parasites, being a possible alternative therapy in the treatment of cutaneous leishmaniosis.
Keywords: Leishmaniosis
PDT
Laser
Macrophage
keywords: Leishmania
PDT
Laser
Macrófagos
Issue Date: 2017
Publisher: Springer Verlag
Citation: OLIVEIRA, S. et al. Leishmanicidal effect of antiparasitic photodynamic therapy-ApPDT on infected macrophages. Lasers in Medical Science, jul. 2017.
DOI: 10.1007/s10103-017-2292-9
ISSN: 0268-8921
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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