Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/20674
Title: The Vasoactive Peptide Maxadilan from Sand Fly Saliva Inhibits TNF-! and Induces IL-6 by Mouse Macrophages Through Interaction with the Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) Receptor
Authors: Soares, Milena Botelho Pereira
Titus, Richard G
Shoemaker, Charles B
David, John R
Bozza, Marcelo Torres
Affilliation: Harvard School of Public Health. Department of Tropical Public Health. Boston, MA, USA
Colorado State University. College of Veterinary Medicine and Biomedical Sciences. Department of Pathology. Fort Collins, Colorado
Harvard School of Public Health. Department of Tropical Public Health. Boston, MA, USA
Harvard School of Public Health. Department of Tropical Public Health. Boston, MA, USA
Harvard School of Public Health. Department of Tropical Public Health. Boston, MA, USA
Abstract: Maxadilan is a vasodilatory peptide encoded by a gene cloned from Lutzomyia longipalpis salivary glands. In this study we investigated the effects of maxadilan on macrophage functions. Maxadilan treatment of LPS-stimulated BALB/c macrophages inhibited TNF-! release but increased IL-6. Further, it also induced IL-6 release in a dose-dependent manner from unstimulated macrophages. Maxadilan increased production of PGE2, and the inhibition of TNF-! was completely abrogated by indomethacin. Others have recently shown that maxadilan is a selective agonist of the pituitary adenylate cyclase-activating polypeptide (PACAP) type I receptor. Treatment with the receptor antagonist PACAP 6–38 blocked maxadilan activities on macrophages. The natural endogenous ligand, PACAP 38, had the same effects as maxadilan on TNF-! and IL-6 production. Finally, in a dose- and time-dependent fashion, maxadilan induced the intracellular accumulation of cAMP in macrophages. Taken together, the results presented here indicate a modulatory effect of ligands of PACAP type I receptor on cytokine production by macrophages and suggest that activation of this receptor, with the subsequent elevation of intracellular cAMP in macrophages, could participate in a negative-feedback mechanism that controls certain inflammatory responses
Keywords: Salivary glands
vasodilatory
Lutzomyia
Treatment
Macrophages
keywords: Glandulas salivares
Vasodilatador
Lutzomyia
Tratamento
Macrófagos
Issue Date: 1998
Publisher: American Association of Immunologists
Citation: SOARES, M. B. P. et al. The Vasoactive Peptide Maxadilan from Sand Fly Saliva Inhibits TNF-! and Induces IL-6 by Mouse Macrophages Through Interaction with the Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) Receptor. Journal of Immunology, v. 160, p. 1811-1816, 1998.
Description: Soares, Milena Botelho Pereira “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.
ISSN: 0022-1767
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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