Title:	Antitumor effect of the essential oil from leaves of Guatteria pogonopus (Annonaceae)
Authors:	Fontes, José Eraldo do Nascimento Ferraz, Rosana Paula Cruz Britto, Anny Caroline Siqueira Carvalho, Adriana Andrade Moraes, Manoel O Pessoa, Claudia do Ó Costa, Emmanoel Vilaça Bezerra, Daniel Pereira
Affilliation:	Federal University of Sergipe. Department of Chemistry. São Cristóvão. Aracaju, SE, Brazil Federal University of Sergipe. Department of Physiology. São Cristóvão. Aracaju, SE, Brazil Federal University of Sergipe. Department of Physiology. São Cristóvão. Aracaju, SE, Brazil Federal University of Ceará. School of Medicine. Department of Physiology and Pharmacology. Fortaleza, CE, Brazil Federal University of Ceará. School of Medicine. Department of Physiology and Pharmacology. Fortaleza, CE, Brazil Federal University of Ceará. School of Medicine. Department of Physiology and Pharmacology. Fortaleza, CE, Brazil Federal University of Sergipe. Department of Chemistry. São Cristóvão. Aracaju, SE, Brazil Federal University of Sergipe. Department of Physiology. São Cristóvão. Aracaju, SE, Brazil
Abstract:	Guatteria pogonopus Martius, a plant belonging to the Annonaceae family, is found in the remaining Brazilian Atlantic Forest. In this study, the chemical composition and antitumor effects of the essential oil isolated from leaves of G. pogonopus was investigated. The chemical composition of the oil was determined by GC-FID and GC/MS analyses. The in vitro cytotoxicity was evaluated against three different tumor cell lines (OVCAR-8, NCI-H358M, and PC-3M), and the in vivo antitumor activity was tested in mice bearing sarcoma 180 tumor. A total of 29 compounds was identified and quantified in the oil. The major compounds were γ-patchoulene (13.55%), (E)-caryophyllene (11.36%), β-pinene (10.37%), germacrene D (6.72%), bicyclogermacrene (5.97%), α-pinene (5.33%), and germacrene B (4.69%). The essential oil, but neither (E)-caryophyllene nor β-pinene, displayed in vitro cytotoxicity against all three tumor cell lines tested. The obtained average IC50 values ranged from 3.8 to 20.8 μg/ml. The lowest and highest values were obtained against the NCI-H358M and the OVCAR-8 cell lines, respectively. The in vivo tumor-growth-inhibition rates in the tumor-bearing mice treated with essential oil (50 and 100 mg/kg/d) were 25.3 and 42.6%, respectively. Hence, the essential oil showed significant in vitro and in vivo antitumor activity.
Keywords:	Sarcoma Cell Line, Tumor Neoplasms Transplantation, Heterologous Plant Leaves Oils, Volatile Mice
keywords:	Sarcoma Tumor Neoplasias Transplante Folhas de plantas Óleos Ratos
Issue Date:	2013
Publisher:	Wiley
Citation:	FONTES, J. E. N. et al. Antitumor effect of the essential oil from leaves of Guatteria pogonopus (Annonaceae). Chemistry and Biodiversity, v. 10, p. 722-729, 2013.
Description:	Bezerra, Daniel Pereira “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.
DOI:	10.1002/cbdv.201200304
ISSN:	1612-1872
Copyright:	open access
Appears in Collections:	BA - IGM - Artigos de Periódicos

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