Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/20816
Title: CCL2/MCP-1 controls parasite burden, cell infiltration, and mononuclear activation during acute Trypanosoma cruzi infection
Authors: Paiva, Claudia N.
Figueiredo, Rodrigo T.
Palhares, Karina Kroll
Silva, Andréa A.
Silvério, Jaline C.
Gibaldi, Daniel
Pyrrho, Alexandre dos S.
Benjamim, Claudia F.
Lannes-Vieira, Joseli
Bozza, Marcelo T.
Affilliation: Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Prof Paulo de Góes. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Prof Paulo de Góes. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Auto-imunidade e Imuno-regulação. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Auto-imunidade e Imuno-regulação. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Auto-imunidade e Imuno-regulação. Rio de Janeiro, RJ. Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Prof Paulo de Góes. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil./ Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Auto-imunidade e Imuno-regulação. Rio de Janeiro, RJ. Brasil.
Universidade Federal do Rio de Janeiro. Faculdade de Farmácia. Departamento de Análises Cínicas e Toxicológicas. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Departamento de Farmacologia Básica e Clínica. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Auto-imunidade e Imuno-regulação. Rio de Janeiro, RJ. Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Prof Paulo de Góes. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil.
Abstract: CCL2/MCP-1 has emerged recently as a critical factor in infectious and autoimmune myocarditis. In fact, this chemokine is produced in great amounts in hearts from Trypanosoma cruzi-infected mice and is known to enhance parasite uptake and destruction by macrophages. Herein, we studied the involvement of CCL2 in tissue inflammation and resistance to T. cruzi. Infected CCL2 / mice developed higher parasitemias and died earlier than WT mice. Close to their death, T. cruzi-infected CCL2 / presented greater amounts of TNF, IFN- , and IL-10 in plasma than WTs and clinical signs of systemic inflammatory response. Amastigote nests were more frequent in hearts and livers from infected CCL2 / tissues than in WTs, and reduced numbers of leukocytes infiltrated their tissues. Leukocytes formed diffuse but not focal infiltrates in hearts from infected CCL2 / mice, and perivascular cuffs could still be found in their livers. Infected CCL2 / mice had smaller percentages of activated CD11b (Mac-1) CD107b (Mac-3) macrophages and CD8 CD69hi cells among heart and liver infiltrates than WTs (flow cytometry), indicating that CCL2 controls subset migration/activation. CCL2 accumulated among focal heart infiltrates, suggesting that this chemokine is involved in retention of mononuclear cells in particular spots. Peritoneal macrophages from CCL2 / mice displayed decreased trypanocidal activity. Our results demonstrate that CCL2 contributes to reduce parasite growth and indicate that it does so by controlling the distribution, cellular composition, and state of activation of inflammatory infiltrates in acute T. cruzi infection.
Keywords: Trypanosoma cruzi
infection
CCL2/MCP-1
Control
keywords: Trypanosoma cruzi
infecção
Controle
Issue Date: 2009
Publisher: Society for Leukocyte Biology
Citation: PAIVA, Cláúdia N. et al. CCL2/MCP-1 controls parasite burden, cell infiltration, and mononuclear activation during acute Trypanosoma cruzi infection. Journal of Leukocyte Biology, v.86, p.1239-1246, Nov. 2009.
ISSN: 0741-5400
Copyright: restricted access
Appears in Collections:IOC - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
karina_palhares_etal_IOC_2009.pdf553.15 kBAdobe PDF    Request a copy


FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.