| Author | Salomão, Kelly | |
| Author | Souza, Eniuce M. de | |
| Author | Pons, Andrea Henriques | |
| Author | Barbosa, Helene S. | |
| Author | Castro, Solange L. de | |
| Access date | 2017-09-05T15:16:52Z | |
| Available date | 2017-09-05T15:16:52Z | |
| Document date | 2011 | |
| Citation | SALOMÂO, Kelly; et al. Brazilian Green Propolis: Effects In Vitro and In Vivo on Trypanosoma cruzi. Evidence-Based Complementary and Alternative Medicine, Volume 2011, Article ID 185918, 11p, 2011. | pt_BR |
| ISSN | 1741-427X | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/20919 | |
| Language | eng | pt_BR |
| Publisher | Hindawi Publishing Corporation | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Trypanosoma cruzi | pt_BR |
| Subject in Portuguese | In vitro | pt_BR |
| Subject in Portuguese | In vivo | pt_BR |
| Subject in Portuguese | Própole | pt_BR |
| Subject in Portuguese | Brasil | pt_BR |
| Title | Brazilian Green Propolis: Effects In Vitro and In Vivo on Trypanosoma cruzi | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1093/ecam/nep014 | |
| Abstract | The composition of a Brazilian green propolis ethanolic extract (Et-Bra) and its effect on Trypanosoma cruzi trypomastigotes and other pathogenic microorganisms have already been reported. Here, we further investigated Et-Bra targets in T. cruzi and its effect on experimental infection of mice. The IC(50)/4 days for inhibition of amastigote proliferation was 8.5 ± 1.8 μg mL(-1), with no damage to the host cells. In epimastigotes Et-Bra induced alterations in reservosomes, Golgi complex and mitochondrion. These effects were confirmed by flow cytometry analysis. In trypomastigotes, Et-Bra led to the loss of plasma membrane integrity. The in vitro studies indicate that Et-Bra interferes in the functionality of the plasma membrane in trypomastigotes and of reservosomes and mitochondrion in epimastigotes. Acutely infected mice were treated orally with Et-Bra and the parasitemia, mortality and GPT, GOT, CK and urea levels were monitored. The extract (25-300 mg kg(-1) body weight/day for 10 days) reduced the parasitemia, although not at significant levels; increased the survival of the animals and did not induce any hepatic, muscular lesion or renal toxicity. Since Et-Bra was not toxic to the animals, it could be assayed in combination with other drugs. Et-Bra could be a potential metacyclogenesis blocker, considering its effect on reservosomes, which are an important energy source during parasite differentiation. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Subject | Trypanosoma cruzi | pt_BR |
| Subject | In vitro | pt_BR |
| Subject | In vivo | pt_BR |
| Subject | Brazilian green propolis | pt_BR |
| Subject | Brazil | pt_BR |
| e-ISSN | 1741-4288 | |
