Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/20936
Title: Performance assessment of a Trypanosoma cruzi chimeric antigen in multiplex liquid microarray assays
Authors: Santos, Fred Luciano Neves
Celedon, Paola Alejandra Fiorani
Zanchin, Nilson Ivo Tonin
Leitolis, Amanda
Crestani, Sandra
Foti, Leonardo
Souza, Wayner Vieira de
Gomes, Yara de Miranda
Krieger, Marco Aurélio
Affilliation: Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Programa Integrado de Doença de Chagas. Rio de Janeiro, RJ, Brasil.
Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.
Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.
Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Programa Integrado de Doença de Chagas. Rio de Janeiro, RJ, Brasil.
Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Programa Integrado de Doença de Chagas. Rio de Janeiro, RJ, Brasil.
Abstract: Diagnosing chronic Chagas disease (CD) requires antibody--antigen detection methods, traditionally based on enzymatic assay techniques whose performance depend on the type and quality of antigen used. Previously, 4 recombinant chimeric proteins from Instituto de Biologia Molecular do Paraná (IBMP-8.1 to -8.4) comprising immuno-dominant regions of diverse Trypanosoma cruzi antigens showed excellent diagnostic performance in enzyme-linked immunosorbent assays. Considering that next-generation platforms offer improved CD diagnostic accuracy with different T. cruzi-specific recombinant antigens, we assessed the performance of these chimeras in liquid microarrays (LMAs). The chimeric proteins were expressed in Escherichia coli and purified by chromatography. Sera from 653 chagasic and 680 healthy individuals were used to assess the performance of these chimeras in detecting specific anti-T. cruzi antibodies. Accuracies ranged from 98.1--99.3%, and diagnostic odds ratio values were 3,548 for IBMP-8.3, 4,826 for IBMP-8.1, 7,882 for IBMP-8.2, and 25,000 for IBMP-8.4. A separate sera bank (851 samples) was employed to assess cross-reactivity with other tropical diseases. Leishmania spp., a pathogen with high genome sequence similar to T. cruzi, showed cross-reactivity rates ranging from 0--2.17%. Inconclusive results were negligible (0--0.71%). Bland--Altman and Deming regression analysis based on 200 randomly selected CD-positive and -negative samples demonstrated interchangeability with respect to CD diagnostic performance in both singleplex and multiplex assays. Our results suggested that these chimeras can potentially replace antigens currently used in commercially available assay kits. Moreover, the use of a multiplex platform, like LMA assays employing 2 or more IBMP antigens, would abrogate the need for 2 different testing techniques when diagnosing CD.
Keywords: Chagas Disease
Mutant Chimeric Proteins
Antigens
Keywords in spanish: Enfermedad de Chagas
Proteínas Mutantes Quiméricas
Antígenos
keywords: Trypanosoma cruzi
DeCS: Doença de Chagas
Proteínas Mutantes Quiméricas
Antígenos
Issue Date: 2017
Publisher: American Society For Microbiology
Citation: SANTOS, Fred Luciano Neves et al. Performance assessment of a Trypanosoma cruzi chimeric antigen in multiplex liquid microarray assays. Journal of Clinical Microbiology, v. 55, n. 10, p. 2934-2945, 2017.
DOI: 10.1128/JCM.00851-17
ISSN: 1098-660X
Copyright: open access
Appears in Collections:PE - IAM - Artigos de Periódicos
BA - IGM - Artigos de Periódicos
IOC - Artigos de Periódicos
PR - ICC - Artigos de Periódicos

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