Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/20979
Title: Involvement of lectin pathway activation in the complement killing of Giardia intestinalis
Authors: Evans-Osses, Ingrid
Ansa-Addo, Ephraim A.
Inal, Jameel M.
Ramirez, Marcel I.
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Parasitas e Vetores. Rio de Janeiro, RJ. Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.
London Metropolitan University. Faculty of Life Sciences. Cellular and Molecular Immunology Research Centre. London, UK.
London Metropolitan University. Faculty of Life Sciences. Cellular and Molecular Immunology Research Centre. London, UK.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Parasitas e Vetores. Rio de Janeiro, RJ. Brasil.
Abstract: Giardia intestinalis (syn. G. lamblia, G. duodenalis) is a flagellated unicellular eukaryotic microorganism that commonly causes diarrheal disease throughout the world. In humans, the clinical effects of Giardia infection range from the asymptomatic carrier state to a severe malabsorption syndrome possibly due to different virulence of the Giardia strain, the number of cysts ingested, the age of the host, and the state of the host immune system at the time of infection. The question about how G. intestinalis is controlled by the organism remains unanswered. Here, we investigated the role of the complement system and in particular, the lectin pathway during Giardia infections. We present the first evidence that G. intestinalis activate the complement lectin pathway and in doing so participate in eradication of the parasite. We detected rapid binding of mannan-binding lectin, H-ficolin and L-ficolin to the surface of G. intestinalis trophozoites and normal human serum depleted of these molecules failed to kill the parasites. Our finding provides insight into the role of lectin pathway in the control of G. intestinalis and about the nature of surface components of parasite.
Keywords: Giardia intestinalis
Lectin pathway
Trophozoites
Complement-mediated killing
Intestinal disease
Mannose
GlcNAc
keywords: Giardia lamblia
Trofozoítos
Manose
Enteropatias
Receptores de N-Acetilglucosamina
Lectina de Ligação
Issue Date: 2010
Publisher: Elsevier
Citation: EVAN-OSSES, Ingrid; et al. Involvement of lectin pathway activation in the complement killing of Giardia intestinalis. Biochemical and Biophysical Research Communications, v.395, p.382–386, 2010.
DOI: 10.1016/j.bbrc.2010.04.025
ISSN: 0006-291X
Copyright: restricted access
Appears in Collections:IOC - Artigos de Periódicos

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