| Author | Ansa-Addo, Ephraim A. | |
| Author | Lange, Sigrun | |
| Author | Stratton, Dan | |
| Author | Antwi-Baffour, Samuel | |
| Author | Cestari, Igor | |
| Author | Ramirez, Marcel I. | |
| Author | McCrossan, Maria V. | |
| Author | Inal, Jameel M. | |
| Access date | 2017-09-12T18:07:27Z | |
| Available date | 2017-09-12T18:07:27Z | |
| Document date | 2010 | |
| Citation | ANSA-ADDO, Ephraim A. et al. Human Plasma Membrane-Derived Vesicles Halt Proliferation and Induce Differentiation of THP-1 Acute Monocytic Leukemia Cells. The Journal of Immunology, v.185, p.5236-5246, Oct. 2010. | pt_BR |
| ISSN | 0022-1767 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/20984 | |
| Language | eng | pt_BR |
| Publisher | American Association of Immunologists | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Vesículas plasmáticas derivadas da membrana | pt_BR |
| Subject in Portuguese | Perda | pt_BR |
| Subject in Portuguese | Leucemia Monocítica Aguda | pt_BR |
| Subject in Portuguese | Induzem a diferenciação de THP-1 | pt_BR |
| Title | Human plasma membrane-derived vesicles halt proliferation and induce differentiation of THP-1 acute monocytic leukemia cells | pt_BR |
| Type | Article | |
| DOI | 10.4049/jimmunol.1001656 | |
| Abstract | Plasma membrane-derived vesicles (PMVs) are small intact vesicles released from the cell surface that play a role in intercellular communication. We have examined the role of PMVs in the terminal differentiation of monocytes. The myeloid-differentiating agents all-trans retinoic acid/PMA and histamine, the inflammatory mediator that inhibits promonocyte proliferation, induced an intracellular Ca(2+)-mediated PMV (as opposed to exosome) release from THP-1 promonocytes. These PMVs cause THP-1 cells to enter G(0)-G(1) cell cycle arrest and induce terminal monocyte-to-macrophage differentiation. Use of the TGF-β receptor antagonist SB-431542 and anti-TGF-β1 Ab showed that this was due to TGF-β1 carried on PMVs. Although TGF-β1 levels have been shown to increase in cell culture supernatants during macrophage differentiation and dendritic cell maturation, the presence of TGF-β1 in PMVs is yet to be reported. In this study, to our knowledge we show for the first time that TGF-β1 is carried on the surface of PMVs, and we confirm the presence within PMVs of certain leaderless proteins, with reported roles in myeloid cell differentiation. Our in vitro findings support a model in which TGF-β1-bearing PMVs, released from promonocytic leukemia cells (THP-1) or primary peripheral blood monocytes on exposure to sublytic complement or after treatment with a differentiation therapy agent, such as all-trans retinoic acid, significantly reduce proliferation of THP-1 cells. Such PMVs also induce the terminal differentiation of primary peripheral blood monocytes as well as THP-1 monocytes. | pt_BR |
| Affilliation | London Metropolitan University. Faculty of Life Sciences. School of Human Sciences. Cellular and Molecular Immunology Research Centre, School of Human Sciences. London, United Kingdom. | pt_BR |
| Affilliation | University College of London Institute for Women’s Health. Maternal and Fetal Medicine. Perinatal Brain Repair Group. London, United Kingdom. | pt_BR |
| Affilliation | London Metropolitan University. Faculty of Life Sciences. School of Human Sciences. Cellular and Molecular Immunology Research Centre, School of Human Sciences. London, United Kingdom. | pt_BR |
| Affilliation | London Metropolitan University. Faculty of Life Sciences. School of Human Sciences. Cellular and Molecular Immunology Research Centre, School of Human Sciences. London, United Kingdom. | pt_BR |
| Affilliation | London Metropolitan University. Faculty of Life Sciences. School of Human Sciences. Cellular and Molecular Immunology Research Centre, School of Human Sciences. London, United Kingdom / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | London Metropolitan University. Faculty of Life Sciences. School of Human Sciences. Cellular and Molecular Immunology Research Centre, School of Human Sciences. London, United Kingdom / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | London School of Hygiene and Tropical Medicine. Immunology Unit. London, United Kingdom. | pt_BR |
| Affilliation | London Metropolitan University. Faculty of Life Sciences. School of Human Sciences. Cellular and Molecular Immunology Research Centre, School of Human Sciences. London, United Kingdom. | pt_BR |
| Subject | Plasma membrane-derived vesicles | pt_BR |
| Subject | Acute Monocytic Leukemia | pt_BR |
| Subject | Induce Differentiation of THP-1 | pt_BR |
| e-ISSN | 1550-6606 | |
| Embargo date | 2030-01-01 | |
