Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/20984
Title: Human plasma membrane-derived vesicles halt proliferation and induce differentiation of THP-1 acute monocytic leukemia cells
Authors: Ansa-Addo, Ephraim A.
Lange, Sigrun
Stratton, Dan
Antwi-Baffour, Samuel
Cestari, Igor
Ramirez, Marcel I.
McCrossan, Maria V.
Inal, Jameel M.
Affilliation: London Metropolitan University. Faculty of Life Sciences. School of Human Sciences. Cellular and Molecular Immunology Research Centre, School of Human Sciences. London, United Kingdom.
University College of London Institute for Women’s Health. Maternal and Fetal Medicine. Perinatal Brain Repair Group. London, United Kingdom.
London Metropolitan University. Faculty of Life Sciences. School of Human Sciences. Cellular and Molecular Immunology Research Centre, School of Human Sciences. London, United Kingdom.
London Metropolitan University. Faculty of Life Sciences. School of Human Sciences. Cellular and Molecular Immunology Research Centre, School of Human Sciences. London, United Kingdom.
London Metropolitan University. Faculty of Life Sciences. School of Human Sciences. Cellular and Molecular Immunology Research Centre, School of Human Sciences. London, United Kingdom / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil
London Metropolitan University. Faculty of Life Sciences. School of Human Sciences. Cellular and Molecular Immunology Research Centre, School of Human Sciences. London, United Kingdom / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil
London School of Hygiene and Tropical Medicine. Immunology Unit. London, United Kingdom.
London Metropolitan University. Faculty of Life Sciences. School of Human Sciences. Cellular and Molecular Immunology Research Centre, School of Human Sciences. London, United Kingdom.
Abstract: Plasma membrane-derived vesicles (PMVs) are small intact vesicles released from the cell surface that play a role in intercellular communication. We have examined the role of PMVs in the terminal differentiation of monocytes. The myeloid-differentiating agents all-trans retinoic acid/PMA and histamine, the inflammatory mediator that inhibits promonocyte proliferation, induced an intracellular Ca(2+)-mediated PMV (as opposed to exosome) release from THP-1 promonocytes. These PMVs cause THP-1 cells to enter G(0)-G(1) cell cycle arrest and induce terminal monocyte-to-macrophage differentiation. Use of the TGF-β receptor antagonist SB-431542 and anti-TGF-β1 Ab showed that this was due to TGF-β1 carried on PMVs. Although TGF-β1 levels have been shown to increase in cell culture supernatants during macrophage differentiation and dendritic cell maturation, the presence of TGF-β1 in PMVs is yet to be reported. In this study, to our knowledge we show for the first time that TGF-β1 is carried on the surface of PMVs, and we confirm the presence within PMVs of certain leaderless proteins, with reported roles in myeloid cell differentiation. Our in vitro findings support a model in which TGF-β1-bearing PMVs, released from promonocytic leukemia cells (THP-1) or primary peripheral blood monocytes on exposure to sublytic complement or after treatment with a differentiation therapy agent, such as all-trans retinoic acid, significantly reduce proliferation of THP-1 cells. Such PMVs also induce the terminal differentiation of primary peripheral blood monocytes as well as THP-1 monocytes.
Keywords: Plasma membrane-derived vesicles
Acute Monocytic Leukemia
Induce Differentiation of THP-1
keywords: Vesículas plasmáticas derivadas da membrana
Perda
Leucemia Monocítica Aguda
Induzem a diferenciação de THP-1
Issue Date: 2010
Publisher: American Association of Immunologists
Citation: ANSA-ADDO, Ephraim A. et al. Human Plasma Membrane-Derived Vesicles Halt Proliferation and Induce Differentiation of THP-1 Acute Monocytic Leukemia Cells. The Journal of Immunology, v.185, p.5236-5246, Oct. 2010.
DOI: 10.4049/jimmunol.1001656
ISSN: 0022-1767
Copyright: restricted access
Appears in Collections:IOC - Artigos de Periódicos

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