| Author | Romero, Lazara | |
| Author | Marcussi, Silvana | |
| Author | Salvador, Daniela P. Marchi | |
| Author | Silva, Floriano P. | |
| Author | Fuly, André L. | |
| Author | Stábeli, Rodrigo G. | |
| Author | Silva, Saulo L. da | |
| Author | González, Jorge | |
| Author | Monte, Alberto Del | |
| Author | Soares, Andreimar M. | |
| Access date | 2017-09-19T15:56:48Z | |
| Available date | 2017-09-19T15:56:48Z | |
| Document date | 2010 | |
| Citation | ROMERO, Lazara; et al. .Enzymatic and structural characterization of a basic phospholipase A2 from the sea anemone Condylactis gigantea. Biochimie, v.92, n.8, p.1063-1071, 2010. | pt_BR |
| ISSN | 0300-9084 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/21061 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Anêmonas de mar | pt_BR |
| Subject in Portuguese | Análise filogenética | pt_BR |
| Subject in Portuguese | Estrutura primária | pt_BR |
| Subject in Portuguese | Caracterização funcional | pt_BR |
| Subject in Portuguese | Fosfolipases A2 | pt_BR |
| Subject in Portuguese | Modelagem molecular | pt_BR |
| Title | Enzymatic and structural characterization of a basic phospholipase A(2) from the sea anemone Condylactis gigantea | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.biochi.2010.05.007 | |
| Abstract | This work aimed at the isolation and structural/functional characterization of a phospholipase A(2) (CgPLA(2)) from the extract of the anemone Condylactis gigantea. CgPLA(2) was isolated with a high purity level through three chromatographic steps, showing pI 8.6 and molecular weights of 14,500 and 29,000 for the monomer and dimer, respectively. CgPLA(2) showed a high catalytic activity upon fluorescent phospholipids inducing no direct hemolytic activity. This enzyme, which is Ca(2+)-dependent, showed a lower stability against temperature and pH variations when compared with snake venom enzymes. The enzymatic activity was significantly reduced or completely abolished after chemical modification of CgPLA(2) with BPB. Its cDNA was then obtained, with 357 base pairs which codified for a mature protein of 119 amino acid residues. A comparative analysis of the primary structure of CgPLA(2) revealed 84%, 61%, 43% and 42% similarity to the PLA(2)s from Adamsia carciniopados, Nematostella vectensis, Vipera russelli russelli and Bothrops jararacussu, respectively. | pt_BR |
| Affilliation | Universidad de La Habana. Facultad de Biologia. Centro de Estudios de Proteínas. Havana, Cuba. | pt_BR |
| Affilliation | Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas. Ribeirão Preto, SP, Brasil./ Universidade Federal de Lavras. Departamento de Química. Lavras, MG, Brasil | pt_BR |
| Affilliation | Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas. Ribeirão Preto, SP, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica de Proteínas e Peptídeos. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Celular e Molecular. Niterói, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Fiocruz Noroeste. Porto |Velho, RO, Brasil / Universidade Federal de Rondonia, UNIR, RO, Brasil. | pt_BR |
| Affilliation | Universidade Federal de São João Del Rei. Divinópolis,MG, Brasil. | pt_BR |
| Affilliation | Universidad de La Habana. Facultad de Biologia. Centro de Estudios de Proteínas. Havana, Cuba. | pt_BR |
| Affilliation | Universidad de La Habana. Facultad de Biologia. Centro de Estudios de Proteínas. Havana, Cuba. | pt_BR |
| Affilliation | Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas. Ribeirão Preto, SP, Brasil. | pt_BR |
| Subject | Sea anemones | pt_BR |
| Subject | Condylactis gigantea | pt_BR |
| Subject | Phospholipase A2 | pt_BR |
| Subject | Primary structure | pt_BR |
| Subject | Functional characterization | pt_BR |
| Subject | Molecular modeling | pt_BR |
| Subject | Phylogenetic analysis | pt_BR |
| e-ISSN | 1638-6183 | |
| Embargo date | 2030-01-01 | |
