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DETECTION OF STREPTOCOCCUS MUTANS GENOMIC DNA IN HUMAN DNA SAMPLES EXTRACTED FROM SALIVA AND BLOOD
Vieira, Alexandre R. et al. | Date Issued: 2011
Author
Vieira, Alexandre R.
Deeley, Kathleen B.
Callahan, Nicholas F.
Noel, Jacqueline B.
Anjomshoaa, Ida
Carricato, Wendy M.
Schulhof, Louise P.
Desensi, Rebecca S.
Gandhi, Pooja
Resick, Judith M.
Brandon, Carla A.
Rozhon, Christopher
Patir, Asli
Yildirim, Mine
Poletta, Fernando A.
Mereb, Juan C.
Letra, Ariadne
Menezes, Renato
Wendell, Steven
López-Camelo, Jorge S.
Castilla, Eduardo E.
Orioli, Iêda M.
Seymen, Figen
Weyant, Robert J.
Crout, Richard
McNeil, Daniel W.
Modesto, Adriana
Marazita, Mary L.
Affilliation
University of Pittsburgh. School of Dental Medicine. Department of Oral Biology. Pittsburgh, PA, USA / University of Pittsburgh. School of Dental Medicine. Department of Pediatric Dentistry, Pittsburg, PA, USA / University of Pittsburgh. School of Dental Medicine; Center for Craniofacial and Dental Genetics. Pittisburg, PA, USA / University of Pittsburgh. Graduate School of Public Health. Department of Human Genetics. Pittsburgh, PA, USA / University of Pittsburgh. Clinical and Translational Science Institute. Pittsburg, PA, USA.
University of Pittsburgh. School of Dental Medicine. Department of Oral Biology. Pittsburgh, PA, USA / University of Pittsburgh. School of Dental Medicine. Center for Craniofacial and Dental Genetics, PA, USA / University of Pittsburgh. Graduate School of Public Health. Department of Human Genetics. Pittsburgh, PA, USA.
University of Pittsburgh. School of Dental Medicine. Department of Oral Biology. Pittsburgh, PA, USA / University of Pittsburgh. School of Dental Medicine. Center for Craniofacial and Dental Genetics, PA, USA
University of Pittsburgh. School of Dental Medicine. Department of Oral Biology. Pittsburgh, PA, USA / University of Pittsburgh. School of Dental Medicine. Center for Craniofacial and Dental Genetics, PA, USA
University of Pittsburgh. School of Dental Medicine. Department of Oral Biology. Pittsburgh, PA, USA / University of Pittsburgh. School of Dental Medicine. Center for Craniofacial and Dental Genetics, PA, USA
University of Pittsburgh. School of Dental Medicine. Department of Oral Biology. Pittsburgh, PA, USA / University of Pittsburgh. School of Dental Medicine. Center for Craniofacial and Dental Genetics, PA, USA
University of Pittsburgh. School of Dental Medicine. Center for Craniofacial and Dental Genetics, PA, USA / University of Pittsburgh.. Dental Public Health/Information Management,. Pittsburgh, PA, USA.
University of Pittsburgh. School of Dental Medicine. Department of Oral Biology. Pittsburgh, PA, USA / University of Pittsburgh. School of Dental Medicine. Center for Craniofacial and Dental Genetics, PA, USA
University of Pittsburgh. School of Dental Medicine. Department of Oral Biology. Pittsburgh, PA, USA / University of Pittsburgh. School of Dental Medicine. Center for Craniofacial and Dental Genetics, PA, USA
University of Pittsburgh. School of Dental Medicine. Department of Oral Biology. Pittsburgh, PA, USA / University of Pittsburgh. School of Dental Medicine. Center for Craniofacial and Dental Genetics, PA, USA
University of Pittsburgh. School of Dental Medicine. Department of Oral Biology. Pittsburgh, PA, USA / University of Pittsburgh. School of Dental Medicine. Center for Craniofacial and Dental Genetics, PA, USA
University of Pittsburgh. School of Dental Medicine. Department of Pediatric Dentistry. Pittsburgh, PA, USA.
Istanbul Medipol University. Department of Pedodontics. Istambul, Turkey.
Istanbul Medipol University. Department of Pedodontics. Istambul, Turkey.
ECLAMC, Center for Medical Education and Clinical Research(CEMIC). Buenos Aires, Argentina / ECLAMC, National Institute of Population Medical Genetics(INAGEMP-CNPq) CEMIC. Buenos Aires, Argentina.
ECLAMC,Hospital de Area El Bolsón, Río Negro, Argentina.
University of Pittsburgh. School of Dental Medicine. Department of Oral Biology. Pittsburgh, PA, USA / University of Pittsburgh. School of Dental Medicine. Center for Craniofacial and Dental Genetics, PA, US
University of Pittsburgh. School of Dental Medicine. Department of Oral Biology. Pittsburgh, PA, USA / University of Pittsburgh. School of Dental Medicine. Center for Craniofacial and Dental Genetics, PA, US
University of Pittsburgh. School of Dental Medicine. Department of Oral Biology. Pittsburgh, PA, USA / University of Pittsburgh. School of Dental Medicine. Center for Craniofacial and Dental Genetics, PA, US
ECLAMC, Center for Medical Education and Clinical Research(CEMIC). Buenos Aires, Argentina / ECLAMC, National Institute of Population Medical Genetics(INAGEMP-CNPq) CEMIC. Buenos Aires, Argentina / ECLAMC, I Multidisciplinary Institute of Cellular Biology (MBICE), La Plata, Argentina.
ECLAMC, Center for Medical Education and Clinical Research(CEMIC). Buenos Aires, Argentina / Fundação Oswaldo Cruz. Departamento de Genética. ECLAMC, National Institute of PopulationMedical Genetics (INAGEMP-CNPq). Rio de Janeiro, RJ, Brasil.
ECLAMC,Hospital de Area El Bolsón, Río Negro, Argentina / Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Biologia. Departamento de Genética. Rio de Janeiro, RJ, Brasil.
Istanbul University. Department of Pedodontics. Istanbul, Turkey.
University of Pittsburgh. Dental Public Health/Information Management.. Pittsburgh, PA, USA.
West Virginia University. School of Dentistry. Department of Periodontics. Morgantown, WV, USA.
West Virginia University. Department of Dental Practice and Rural Health. Morgantown, WY, USA.
University of Pittsburgh. School of Dental Medicine. Department of Pediatric Dentistry, Pittsburg, PA, USA.
Abstract
Caries is a multifactorial disease, and studies aiming to unravel the factors modulating its etiology must consider all known predisposing factors. One major factor is bacterial colonization, and Streptococcus mutans is the main microorganism associated with the initiation of the disease. In our studies, we have access to DNA samples extracted from human saliva and blood. In this report, we tested a real-time PCR assay developed to detect copies of genomic DNA from Streptococcus mutans in 1,424 DNA samples from humans. Our results suggest that we can determine the presence of genomic DNA copies of Streptococcus mutans in both DNA samples from caries-free and caries-affected individuals. However, we were not able to detect the presence of genomic DNA copies of Streptococcus mutans in any DNA samples extracted from peripheral blood, which suggests the assay may not be sensitive enough for this goal. Values of the threshold cycle of the real-time PCR reaction correlate with higher levels of caries experience in children, but this correlation could not be detected for adults.
Keywords in Portuguese
Streptococcus mutans
Reação em Cadeia da Polimerase
Cárie Dentária
amostras de DNA humano
Saliva
Sangue
 
Keywords
human DNA samples
saliva
PCR
Streptococcus mutans
caries
blood
 
Publisher
Hindawi Publishing Corporation
Citation
VIEIRA, Alexandre R. et al. Detection of Streptococcusmutans Genomic DNA in Human DNA Samples Extracted from Saliva and Blood. ISRN Dentistry, Volume 2011, Article ID 543561, 6p, 2011.
DOI
10.5402/2011/543561
ISSN
2090-4371
Notes
International Scholarly Research Network