Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/22875
Title: Kinetoplastid membrane protein-11 is present in promastigotes and amastigotes of Leishmania amazonensis and its surface expression increases during metacyclogenesis
Authors: Matos, Denise C. S.
Faccioli, Lanuza A. P.
Finkelstein, Léa Cysne
Luca, Paula Mello de
Corte-Real, Suzana
Armôa, Geraldo R. G.
Lemes, Elezer Monte Blanco
Ricardo, Débora Decote
Mendonça, Sergio C. F.
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Tecnologia Recombinante. Rio de Janeiro, RJ. Brasil.
Fundação Ooswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Laboratório de Tecnologia Recombinante. Rio de Janeiro, RJ, Brasil.
Fundação Ooswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Laboratório de Tecnologia Recombinante. Rio de Janeiro, RJ, Brasil.
Universidade Federal Rural do Rio de Janeiro. Instituto de Veterinária. Departamento de Microbiologia e Imunologia. Seropédica, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ. Brasil.
Abstract: Kinetoplastid membrane protein-11 (KMP-11), a protein present in all kinetoplastid protozoa, is considered a potential candidate for a leishmaniasis vaccine. A suitable leishmaniasis vaccine candidate molecule must be expressed in amastigotes, the infective stage for mammals. However, the expression of KMP-11 in Leishmania amastigotes has been a subject of controversy. We evaluated the expression of this molecule in logarithmic and stationary growth phase promastigotes, as well as in amastigotes, of Leishmania amazonensis by immunoblotting, flow cytometry and immunocytochemistry, using a monoclonal antibody against KMP-11. We found that KMP-11 is present in promastigotes and amastigotes. In both stages, the protein was found in association with membrane structures (at the cell surface, flagellar pocket and intracellular vesicles). More importantly, its surface expression is higher in amastigotes than in promastigotes and increases during metacyclogenesis. The increased expression of KMP-11 in metacyclic promastigotes, and especially in amastigotes, indicates a role for this molecule in the parasite relationship with the mammalian host. The presence of this molecule in amastigotes is consistent with the previously demonstrated immunoprotective capacity of vaccine prototypes based on the KMP-11-coding gene and the presence of humoral and cellular immune responses to KMP-11 in Leishmania-infected humans and animals.
Keywords: Leishmania amazonensis
KMP-11
expression
amastigote
promastigote
metacyclogenesis
keywords: Leishmaniose
metaciclogênese
Expressão
Proteínas de Membrana
Issue Date: 2010
Publisher: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
Citation: MATOS, Denise C. S. et al. Kinetoplastid membrane protein-11 is present in promastigotes and amastigotes of Leishmania amazonensis and its surface expression increases during metacyclogenesis. Mem Inst Oswaldo Cruz, v.105, n.3, p.341-347, May 2010.
ISSN: 0074-0276
Copyright: open access
Appears in Collections:Biomanguinhos - Artigos de Periódicos
IOC - Artigos de Periódicos

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