Author | Magalhães, Franklin Barbalho | |
Author | Castro Neto, Artur Leonel de | |
Author | Nascimento, Marilia B | |
Author | Santos, Wagner José Tenório dos | |
Author | Medeiros, Zulma Maria de | |
Author | Lima Neto, Adelino Soares | |
Author | Costa, Dorcas Lamounier | |
Author | Costa, Carlos H N | |
Author | dosSantos, Washington Luis Conrado | |
Author | Pontes de Carvalho, Lain Carlos | |
Author | Oliveira, Geraldo Gileno de Sá | |
Author | Melo Neto, Osvaldo Pompilio de | |
Access date | 2017-10-25T13:04:19Z | |
Available date | 2017-10-25T13:04:19Z | |
Document date | 2017 | |
Citation | MAGALHÃES, F. B. et al. Evaluation of a new set of recombinant antigens for the serological diagnosis of human and canine visceral leishmaniasis. Plos One, sep. 2017. | pt_BR |
ISSN | 1932-6203 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/22955 | |
Sponsorship | Brazilian funding agency CNPq and the Brazilian Ministry of Health (Grant MCTI/CNPq/MS-SCTIENo.: 404259/2012). | pt_BR |
Language | eng | pt_BR |
Publisher | Public Library of Science | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Leishmaniasis, Visceral | pt_BR |
Subject in Portuguese | Leishmania infantum | pt_BR |
Subject in Portuguese | Proteínas Recombinantes | pt_BR |
Subject in Portuguese | Antígenos, Protozoários | pt_BR |
Subject in Portuguese | Seqüência de aminoácidos | pt_BR |
Subject in Portuguese | Animais | pt_BR |
Title | Evaluation of a new set of recombinant antigens for the serological diagnosis of human and canine visceral leishmaniasis | pt_BR |
Type | Article | pt_BR |
DOI | 10.1371/journal.pone.0184867 | |
Abstract | Current strategies for the control of zoonotic visceral leishmaniasis (VL) rely on its efficient diagnosis in both human and canine hosts. The most promising and cost effective approach is based on serologic assays with recombinant proteins. However, no single antigen has been found so far which can be effectively used to detect the disease in both dogs and humans. In previous works, we identified Leishmania infantum antigens with potential for the serodiagnosis of VL. Here, we aimed to expand the panel of the available antigens for VL diagnosis through another screening of a genomic expression library. Seven different protein-coding gene fragments were identified, five of which encoding proteins which have not been previously studied in Leishmania and rich in repetitive motifs. Poly-histidine tagged polypeptides were generated from six genes and evaluated for their potential for diagnosis of VL by ELISA (Enzyme Linked ImmunoSorbent Assay) with sera from infected humans and dogs. None of those was valid for the detection of human VL (26-52% sensitivity) although their performance was increased in the canine sera (48-91% sensitivity), with one polypeptide useful for the diagnosis of canine leishmaniasis. Next, we assayed a mixture of three antigens, found to be best for human or canine VL, among 13 identified through different screenings. This "Mix" resulted in similar levels of sensitivity for both human (84%) and canine (88%) sera. With improvements, this validates the use of multiple proteins, including antigens identified here, as components of a single system for the diagnosis of both forms of leishmaniasis. | pt_BR |
Affilliation | Associação Caruaruense de Ensino Superior e Técnico. Caruaru, PE, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Recife, PE, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Recife, PE, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Recife, PE, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Recife, PE, Brasil | pt_BR |
Affilliation | Instituto de Doenças Tropicais Natan Portella. Teresina, PI, Brasil | pt_BR |
Affilliation | Instituto de Doenças Tropicais Natan Portella. Teresina, PI, Brasil | pt_BR |
Affilliation | Instituto de Doenças Tropicais Natan Portella. Teresina, PI, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Recife, PE, Brasil | pt_BR |
Subject | Leishmaniasis, Visceral | pt_BR |
Subject | Leishmania infantum | pt_BR |
Subject | Recombinant Proteins | pt_BR |
Subject | Antigens, Protozoan | pt_BR |
Subject | Amino Acid Sequence | pt_BR |
Subject | Animals | pt_BR |