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EVALUATION OF A NEW SET OF RECOMBINANT ANTIGENS FOR THE SEROLOGICAL DIAGNOSIS OF HUMAN AND CANINE VISCERAL LEISHMANIASIS
Leishmania infantum
Proteínas Recombinantes
Antígenos, Protozoários
Seqüência de aminoácidos
Animais
Leishmania infantum
Recombinant Proteins
Antigens, Protozoan
Amino Acid Sequence
Animals
Author
Magalhães, Franklin Barbalho
Castro Neto, Artur Leonel de
Nascimento, Marilia B
Santos, Wagner José Tenório dos
Medeiros, Zulma Maria de
Lima Neto, Adelino Soares
Costa, Dorcas Lamounier
Costa, Carlos H N
dosSantos, Washington Luis Conrado
Pontes de Carvalho, Lain Carlos
Oliveira, Geraldo Gileno de Sá
Melo Neto, Osvaldo Pompilio de
Castro Neto, Artur Leonel de
Nascimento, Marilia B
Santos, Wagner José Tenório dos
Medeiros, Zulma Maria de
Lima Neto, Adelino Soares
Costa, Dorcas Lamounier
Costa, Carlos H N
dosSantos, Washington Luis Conrado
Pontes de Carvalho, Lain Carlos
Oliveira, Geraldo Gileno de Sá
Melo Neto, Osvaldo Pompilio de
Affilliation
Associação Caruaruense de Ensino Superior e Técnico. Caruaru, PE, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Recife, PE, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Recife, PE, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Recife, PE, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Recife, PE, Brasil
Instituto de Doenças Tropicais Natan Portella. Teresina, PI, Brasil
Instituto de Doenças Tropicais Natan Portella. Teresina, PI, Brasil
Instituto de Doenças Tropicais Natan Portella. Teresina, PI, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Recife, PE, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Recife, PE, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Recife, PE, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Recife, PE, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Recife, PE, Brasil
Instituto de Doenças Tropicais Natan Portella. Teresina, PI, Brasil
Instituto de Doenças Tropicais Natan Portella. Teresina, PI, Brasil
Instituto de Doenças Tropicais Natan Portella. Teresina, PI, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Recife, PE, Brasil
Abstract
Current strategies for the control of zoonotic visceral leishmaniasis (VL) rely on its efficient diagnosis in both human and canine hosts. The most promising and cost effective approach is based on serologic assays with recombinant proteins. However, no single antigen has been found so far which can be effectively used to detect the disease in both dogs and humans. In previous works, we identified Leishmania infantum antigens with potential for the serodiagnosis of VL. Here, we aimed to expand the panel of the available antigens for VL diagnosis through another screening of a genomic expression library. Seven different protein-coding gene fragments were identified, five of which encoding proteins which have not been previously studied in Leishmania and rich in repetitive motifs. Poly-histidine tagged polypeptides were generated from six genes and evaluated for their potential for diagnosis of VL by ELISA (Enzyme Linked ImmunoSorbent Assay) with sera from infected humans and dogs. None of those was valid for the detection of human VL (26-52% sensitivity) although their performance was increased in the canine sera (48-91% sensitivity), with one polypeptide useful for the diagnosis of canine leishmaniasis. Next, we assayed a mixture of three antigens, found to be best for human or canine VL, among 13 identified through different screenings. This "Mix" resulted in similar levels of sensitivity for both human (84%) and canine (88%) sera. With improvements, this validates the use of multiple proteins, including antigens identified here, as components of a single system for the diagnosis of both forms of leishmaniasis.
Keywords in Portuguese
Leishmaniasis, VisceralLeishmania infantum
Proteínas Recombinantes
Antígenos, Protozoários
Seqüência de aminoácidos
Animais
Keywords
Leishmaniasis, VisceralLeishmania infantum
Recombinant Proteins
Antigens, Protozoan
Amino Acid Sequence
Animals
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