First genetic characterization of Fasciola hepatica in Argentina by nuclear and mitochondrial gene markers

Instituto Nacional de Enfermedades Infecciosas “Dr. Carlos G. Malbrán”.Buenos Aires, Argentina / Consejo Nacional de Investigaciones Científicas y Técnicas. Buenos Aires, Argentina.
Universidad Nacional de Catamarca. Facultad de Ciencias de la Salud. San Fernando del Valle de Catamarca, Argentina.
Instituto Nacional de Enfermedades Infecciosas “Dr. Carlos G. Malbrán”.Buenos Aires, Argentina.
Universidad Nacional de Catamarca. Facultad de Ciencias de la Salud. San Fernando del Valle de Catamarca, Argentina.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Helmintos Parasitos de Vertebrados. Rio de Janeiro, RJ. Brasil.
Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Departamento de Microbiologia, Imunologia e Parasitologia. Laboratório de Helmintologia Romero Lascasas Porto. Rio de Janeiro, RJ, Brasil.
Hospital Municipal de Infecciosas “Dr. Francisco Javier Muñiz”. Buenos Aires, Argentiina.
Consejo Nacional de Investigaciones Científicas y Técnicas. Buenos Aires, Argentina / Instituto de Microbiología y Parasitología Médica. Buenos Aires, Argentina.

Abstract

Fasciola hepatica is a trematode showing genetic variation among isolates from different regions of the world. The objective of this work was to characterize for the first time F. hepatica isolates circulating in different regions of Argentina. Twenty-two adult flukes were collected from naturally infected bovine livers in different areas from Argentina and used for DNA extraction. We carried out PCR amplification and sequence analysis of the ribosomal internal transcribed spacer 1 (ITS1), mitochondrial nicotinamide adenine dinucleotide dehydrogenase subunits 4 and 5 (nad4 and nad5) and mitochondrial cytochrome c oxidase subunit I (cox1) genes as genetic markers. Phylogenies were reconstructed using maximum parsimony algorithm. A total of 6 haplotypes were found for cox1, 4 haplotypes for nad4 and 3 haplotypes for nad5. The sequenced ITS1 fragment was identical in all samples. The analyzed cox1 gene fragment is the most variable marker and is recommended for future analyses. No geographic association was found in the Argentinean samples.

Keywords in Portuguese

Fasciola hepatica
Análise filogenética

Keywords

Phylogenetic analysis
Fasciola hepatica
ITS1
nad4
nad5
cox1

Publisher

Elsevier

Citation

CARNEVALE, Silvana; et al. First genetic characterization of Fasciola hepatica in Argentina by nuclear and mitochondrial gene markers. Veterinary Parasitology, v.245, p.34-38, 2017.

DOI

10.1016/j.vetpar.2017.08.006

ISSN

0304-4017

Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/23092

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Copyright

Restricted access

Embargo date

2030-01-01

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