Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/23162
Title: Structural investigation of C6/36 and Vero cell cultures infected with a Brazilian Zika virus
Authors: Vieira, Debora Ferreira Barreto
Jácome, Fernanda Cunha
Silva, Marcos Alexandre Nunes da
Caldas, Gabriela Cardoso
Filippis, Ana Maria Bispo de
Sequeira, Patrícia Carvalho de
Souza, Elen Mello de
Andrade, Audrien Alves
Manso, Pedro Paulo de Abreu
Trindade, Gisela Freitas
Lima, Sheila Maria Barbosa
Barth, Ortrud Monika
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ. Brasil .
Fundação Oswaldo Cruz. Bio-Manguinhos. Laboratório de Tecnologia Virológica. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Bio-Manguinhos. Laboratório de Tecnologia Virológica. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ. Brasil.
Abstract: Zika virus (ZIKV) is a member of the flavivirus genus, and its genome is approximately 10.8 kilobases of positive-strand RNA enclosed in a capsid and surrounded by a membrane. Studies on the replication dynamics of ZIKV are scarce, which limits the development of antiviral agents and vaccines directed against ZIKV. In this study, Aedes albopictus mosquito lineage cells (C6/36 cells) and African green monkey kidney epithelial cells (Vero cells) were inoculated with a ZIKV sample isolated from a Brazilian patient, and the infection was characterized by immunofluorescence staining, phase contrast light microscopy, transmission electron microscopy and real-time RT-PCR. The infection was observed in both cell lineages, and ZIKV particles were observed inside lysosomes, the rough endoplasmic reticulum and viroplasm-like structures. The susceptibility of C6/36 and Vero cells to ZIKV infection was demonstrated. Moreover, this study showed that part of the replicative cycle may occur within viroplasm-like structures, which has not been previously demonstrated in other flaviviruses.
Keywords: Zika virus
Brazil
Vero cells
C6/36 cells
structural investigation
keywords: Zika Virus
Brasil
Células Vero
Pesquisa
Issue Date: 2017
Publisher: Public Library of Science
Citation: VIEIRA, Debora Ferreira Barreto; et al. Structural investigation of C6/36 and Vero cell cultures infected with a Brazilian Zika virus. Plos One, v.12, n.9, e0184397, 18p, Sept. 2107.
DOI: 10.1371/journal.pone.0184397
ISSN: 1932-6203
Copyright: open access
Appears in Collections:Biomanguinhos - Artigos de Periódicos
IOC - Artigos de Periódicos

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