| Author | Assis, Andrêssa S. F. | |
| Author | Otenio, Marcelo Henrique | |
| Author | Drumond, Betânia P. | |
| Author | Fumian, Tulio M. | |
| Author | Miagostovich, Marize P. | |
| Author | Silva, Maria Luzia da Rosa e | |
| Access date | 2017-11-14T14:52:18Z | |
| Available date | 2017-11-14T14:52:18Z | |
| Document date | 2017 | |
| Citation | ASSIS, Andrêssa S. F. et al. Optimization of the skimmed-milk flocculation method for recovery of adenovirus from sludge. Science of the Total Environment, n.583, p.163-168, 2017. | pt_BR |
| ISSN | 0048-9697 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/23179 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Reação em Cadeia da Polimerase em Tempo Real | pt_BR |
| Subject in Portuguese | Adenovírus Humanos | pt_BR |
| Subject in Portuguese | Floculação orgânica | pt_BR |
| Subject in Portuguese | Estação de tratamento de águas residuais | pt_BR |
| Subject in Portuguese | Controle interno | pt_BR |
| Subject in Portuguese | Lodo de esgoto | pt_BR |
| Title | Optimization of the skimmed-milk flocculation method for recovery of adenovirus from sludge | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.scitotenv.2017.01.045 | |
| Abstract | Return of treated sludge to the environment poses concerns and has stimulated the development of studies on viral monitoring in this matrix, in order to assess its potential risks for public health. Human adenovirus (HAdV) has been identified as a putative viral marker of faecal contamination due to its stability and resistance to the sewage treatment process. The aim of this study was to optimize the organic flocculation procedure in order to establish an appropriate methodology for HAdV recovery from sewage sludge samples. Four protocols (A-D) have been proposed, with changes in the initial sample dilution, in the stirring time and in the final concentration of skimmed-milk. A single sludge sample was obtained in Wastewater Treatment Plant (WWTP) and divided into aliquots. In each protocol, three aliquots were inoculated with HAdV and bacteriophage PP7 and a non-inoculated one was used as negative control. Viral load and recovery rate were determined by quantitative PCR. HAdV recovery rate varied between the protocols tested (p=0.016) and the best result was obtained through the protocol C. In order to confirm this result a field study with activated, thickened and digested sludge samples was carried out. Different types of sludge were obtained in two WWTPs and processed using protocol C. HAdV was detected in all samples, with a similar or higher viral load than those obtained with other concentration techniques already applied to sludge. Protocol C proved to be really efficient, with the advantage of showing low cost and practicability in routine laboratories. | pt_BR |
| Affilliation | Universidade Federal de Juiz de Fora. Instituto de Ciências Biológicas. Departamento de Microbiologia. Laboratório de Virologia. Juiz de Fora. MG, Brasil. | pt_BR |
| Affilliation | Embrapa Gado de Leite. Juiz de Fora, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Juiz de Fora. Instituto de Ciências Biológicas. Departamento de Microbiologia. Laboratório de Virologia. Juiz de Fora. MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Universidade Federal de Juiz de Fora. Instituto de Ciências Biológicas. Departamento de Microbiologia. Laboratório de Virologia. Juiz de Fora. MG, Brasil. | pt_BR |
| Subject | Sewage sludge | pt_BR |
| Subject | Wastewater treatment plant | pt_BR |
| Subject | Organic flocculation | pt_BR |
| Subject | Human adenovirus | pt_BR |
| Subject | Internal control | pt_BR |
| Subject | Quantitative PCR | pt_BR |
| e-ISSN | 1879-1026 | |
| Embargo date | 2030-01-01 | |
| xmlui.metadata.dc.subject.ods | 06 Água potável e saneamento | |
